Diaporone A (1), one new dihydroisocoumarin derivative and four known α-dibenzopyrones, alternariol (2), 5'-hydroxyalternariol (3), alternariol 4,10-dimethyl ether (4), and alternariol 4-methyl ether (5) were isolated from the crude extract of the plant endophytic fungus Diaporthe sp. Their structures were determined on the basis of spectroscopic analysis, including 1D and 2D NMR techniques as well as HRESIMS and comparison with data from the literature. The absolute configuration of 1 was assigned by electronic circular dichroism (ECD) calculations. Compound 1 showed moderate antibacterial activity against Bacillus subtilis with the MIC value of 66.7 μM, and exhibited weak cytotoxicity against human cervical carcinoma (HeLa) cell line with IC50 value of 97.4 μM.

中文翻译：

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diaporone A，一种来自植物内生真菌Diaporthe sp。的新型抗菌次生代谢产物。

diaporone A（1），一种新的二氢异香豆素衍生物和四种已知的α-二苯并吡喃酮，Alternariol（2），5'-羟基十八烷醇（3），Alternariol 4,10-二甲基醚（4）和Alternariol 4-甲基醚（5）从植物内生真菌Diaporthe sp。的粗提物中分离得到。它们的结构是在光谱分析的基础上确定的，包括1D和2D NMR技术以及HRESIMS，并与文献数据进行了比较。1的绝对构型由电子圆二色性（ECD）计算指定。化合物1显示出对枯草芽孢杆菌的中等抗菌活性，MIC值为66.7μM，并且对人宫颈癌（HeLa）细胞系的细胞毒性较弱，IC50值为97.4μM。