This Article describes a method that increases the chemical shift dispersion of signals in proton nuclear magnetic resonance (¹H NMR) spectra of peptides by the addition of salts to the sample. We demonstrate that the addition of potassium phosphate to aliquots of reactions of glycine peptides permits the measurement of their rate constants for hydrolysis by a 60 MHz benchtop instrument, which would otherwise be infeasible due to overlapping signals in the salt-free mixtures. The method is described in detail and validated by comparison to analysis on a 400 MHz spectrometer. The ability to use benchtop NMR spectroscopy to study reactions of simple peptides enables interested scientists at a broader array of institutions—not just those institutions capable of affording high-field NMR instruments—to participate in original research projects like those aimed at elucidating the chemistry that led to the origin of life on Earth.

中文翻译：

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盐诱导的化学位移分散促成的益生元相关肽反应的台式NMR光谱

本文介绍了一种在质子核磁共振中增加信号化学位移弥散的方法（¹通过将盐添加到样品中得到的肽的1 H NMR）光谱。我们证明，向甘氨酸肽反应的等分试样中添加磷酸钾可以通过60 MHz台式仪器测量其水解速率常数，否则由于无盐混合物中的信号重叠而无法实现。通过与400 MHz光谱仪上的分析进行比较，对该方法进行了详细说明和验证。利用台式NMR光谱学研究简单肽的反应的能力，使感兴趣的科学家在更广泛的机构中（不仅仅是那些能够提供高磁场NMR仪器的机构）参与到旨在阐明化学反应的原始研究项目中，导致了地球生命的起源。