Solid-phase synthesis is the method of choice for peptide preparation in both research and industrial settings. The whole synthetic process is governed by the initial functionalization of the resin. Although the literature provides several methods to determine such functionalization, the addition of an Fmoc-amino acid and the posterior spectrophotometric measurement of the dibenzofulvene adduct formed after Fmoc removal is the most widely used for this purpose. However, a range of molar extinction coefficient (ε) values and even wavelengths are currently used in the field, with no standardization of the method. Here, we propose a single-point standardization method that involves a standard solution of the corresponding amino acid to be checked that is prepared freshly at the time of the analysis.

中文翻译：

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使用基于Fmoc测定的标准化方法计算固相肽合成中的树脂功能化。

固相合成是研究和工业环境中肽制备的选择方法。整个合成过程取决于树脂的初始功能化。尽管文献提供了几种确定这种官能化的方法，但为此目的，最广泛使用的是添加Fmoc-氨基酸和后分光光度法测量Fmoc去除后形成的二苯并富勒烯加合物。然而，目前在该领域中使用了摩尔消光系数（ε）值和甚至波长的范围，而该方法没有标准化。在这里，我们提出了一种单点标准化方法，该方法涉及在分析时新鲜制备的待检查的相应氨基酸的标准溶液。