Geckos possess strong adhesion ability, even can climb on smooth surface. Previous studies have shown that the setae of geckos play a crucial role in their ability to climb on vertical walls. But the biological molecular mechanism of their adhesion ability remains unclear. In the present study, the expression patterns of corneous beta proteins (CBPs) genes related to claws, scales, and feathers development (named as ge-gprp-9, ge-gprp-10, ge-gprp-11, ge-gprp-12, ge-gprp-13, ge-gprp-14, ge-gprp-15, and ge-gprp-16 respectively) in the developing pad lamellae of different embryonic stages (stage 34, stage 36, stage 39, and stage 42) of the Japanese gecko Gekko japonicus were detected using fluorescence quantitative PCR approach. The results showed that there were significant up-regulated expression of CBPs mRNA at embryonic stage 39 with the embryonic continuous maturation and the highest expression level was detected at embryonic stage 39 or stage 42. Moreover, the expression levels of four CBPs genes ge-gprp-9, ge-gprp-10, ge-gprp-11, and ge-gprp-12 in the embryonic and adult development of gecko were detected by fluorescence in situ hybridization technique. The results from in situ hybridization detection revealed that the positive signals of these CBPs genes expression were the same in the developing pad lamellae of G. japonicus. The positive signals of eight CBPs genes were mainly found in the setae tissue, oberhautchen, and β layer, which suggests these CBPs genes are involved in the growth of setae.

中文翻译：

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在日本壁虎（Gekko japonicus）的发育中，特定角质β蛋白的表达表明了它们在粘着性刚毛的生长中的作用。

壁虎具有很强的附着力，甚至可以爬在光滑的表面上。先前的研究表明，壁虎的刚毛对它们在垂直墙壁上的攀爬能力起着至关重要的作用。但其粘附能力的生物学分子机制仍不清楚。在本研究中，与爪，鳞片和羽毛发育相关的角质β蛋白（CBPs）基因的表达模式（命名为ge-gprp-9，ge-gprp-10，ge-gprp-11，ge-gprp-图12中的ge-gprp-13，ge-gprp-14，ge-gprp-15和ge-gprp-16）分别处于不同胚胎阶段（阶段34，阶段36，阶段39和阶段42）的发育板中使用荧光定量PCR方法检测日本壁虎壁虎。结果表明，随着胚胎的持续成熟，CBPs mRNA在胚胎第39阶段的表达明显上调，在胚胎第39或第42阶段的表达水平最高。另外，四个CBPs基因ge-gprp的表达水平通过荧光原位杂交技术检测壁虎的胚胎和成年发育中的-9，ge-gprp-10，ge-gprp-11和ge-gprp-12。原位杂交检测的结果表明，这些CBPs基因表达的阳性信号在日本假单胞菌的发育板中是相同的。八个CBPs基因的阳性信号主要存在于刚毛的组织，上层和β层，这说明这些CBPs基因参与了刚毛的生长。