Tetranychus cinnabarinus (Boisduval) is a major pest that harms crops and vegetables worldwide. Scopoletin is a promising acaricidal compound. Our previous study demonstrated that the acaricidal mechanism of scopoletin may be via disrupting intracellular Ca2+ homeostasis and calcium signaling pathway. However, the mechanism underlying the specific target location of scopoletin against T. cinnabarinus remains unclear. In this study, the full-length cDNA of the calcium channel (TcT-VDCC) gene from T. cinnabarinus was cloned and characterized. The TcT-VDCC gene is expressed at all developmental stages of T. cinnabarinus but is highly expressed in the larval and nymphal stages. The TcT-VDCC gene was significantly upregulated after treatment with scopoletin, and the RNAi method was used to feed the dsRNA in T. cinnabarinus to silence the TcT-VDCC gene and reduce its sensitivity to scopoletin. The results showed that the acaricidal mechanism of scopoletin on T. cinnabarinus may be related to TcT-VDCC.

中文翻译：

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沉默T型电压门控钙通道基因可降低四叶朱砂（Boisduval）对大pole碱的敏感性。

Tetranychus cinnabarinus（Boisduval）是一种主要害虫，危害全世界的农作物和蔬菜。co草素是一种有前途的杀螨剂。我们以前的研究表明，pole草碱的杀螨机制可能是通过破坏细胞内Ca2 +稳态和钙信号传导途径来实现的。然而，东pole碱对朱砂T.的特定目标位置的潜在机制尚不清楚。在这项研究中，克隆和鉴定了朱砂毛虫钙通道（TcT-VDCC）基因的全长cDNA。TcT-VDCC基因在朱砂线虫的所有发育阶段均表达，但在幼虫和若虫阶段均高度表达。大麦草碱处理后，TcT-VDCC基因显着上调，并且使用RNAi方法在T细胞中补充dsRNA。朱砂使TcT-VDCC基因沉默并降低其对scopoletin的敏感性。结果表明，草素对朱砂叶螨的杀螨作用可能与TcT-VDCC有关。