Background: Inhalatory anesthetics may impact spermatogenesis and sexual behavior. Comprehensive evaluation should be conducted to screen the effect of inhalatory anesthetics on the sperm and semen quality. This experimental research was organized to assess the impacts of sevoflurane during the period of neonatal spermatogenesis. Materials and methods: Twenty-one pregnant mice were obtained from the Pasteur Institute. After birth, neonates were categorized based on exposure to Monitored Anesthesia Care (MAC) of sevoflurane into three groups: experimental 1, experimental 2 and control. In order to investigate the testicular condition, a histological evaluation, including apoptosis study and immunohistochemistry, was performed. Not only apoptotic target genes such as Bax and Bcl-2, but also microRNA17-92, were investigated in testicular samples via real-time polymerase chain reaction (real-time PCR). Results: The outcomes of this work indicated the effects of sevoflurane on spermatogonial and germ cells in testicular tissue via stimulating apoptotic target genes and microRNA-17-92. The proportion of Bax/Bcl-2 in the experimental group was 8.318699 ± 1.093, and the proportion of Bax/Bcl-2 in the control group was 2.631 ± 0.079. There was a significant (p ≤ 0.002) difference among the control group and both experimental groups. Conclusion: Sequential sevoflurane exposure during the neonatal period may create testicular dysfunction due to the high level of apoptosis in spermatogonial cells. Also, sevoflurane may affect spermatogenesis by influencing other biomarkers, such as microRNA.

中文翻译：

---

新生雄性小鼠睾丸生殖细胞上七氟醚麻醉剂暴露的新评估。

背景：吸入麻醉药可能会影响精子发生和性行为。应进行综合评估，以筛选吸入麻醉药对精子和精液质量的影响。进行这项实验研究是为了评估七氟醚在新生儿精子发生期间的影响。材料和方法：从巴斯德研究所获得二十一只怀孕的小鼠。出生后，根据对七氟醚的监测麻醉护理（MAC）的接触情况，将新生儿分为三组：实验1，实验2和对照组。为了调查睾丸状况，进行了包括细胞凋亡研究和免疫组织化学在内的组织学评估。不仅凋亡靶基因如Bax通过实时聚合酶链反应（real-time PCR）在睾丸样本中研究了Bcl-2和Bcl-2，还有microRNA17-92。结果：这项工作的结果表明七氟醚通过刺激细胞凋亡的靶基因和microRNA-17-92对睾丸的精原细胞和生殖细胞产生影响。的比例Bax蛋白/ Bcl-2的实验组为8.318699±1.093，和比例Bax蛋白/ Bcl-2的对照组中为2.631±0.079。有一个显著（p ≤0.002）对照组和两个实验组之间的差异。结论：由于精原细胞中高水平的细胞凋亡，在新生儿期顺序接触七氟醚可能导致睾丸功能障碍。同样，七氟醚可能会通过影响其他生物标记物（例如microRNA）来影响精子发生。