Abstract A novel enzyme-linked aptamer assay is reported for the determination of aflatoxin B1 (AFB1). AFB1 can competitively bind with the immobilized biotin-aptamer and release biotin complementary DNA, leading to the gradual fading of the detection system color with increasing of AFB1 concentration. In the absence of AFB1, the biotinylated complementary DNA is not be released from the fixed aptamer. Therefore, the enzyme reaction occurs in the detection system. Under the optimized experimental conditions, the proposed method possessed a wide linear range for AFB1 from 1 to 80 ng/mL (R2 of 0.990) with a low detection limit of 0.36 ng/mL. The method was then applied to detect uncontaminated peanuts fortified with different concentrations of AFB1. The recovery values were from 82.60% to 94.43%, which indicated the proposed method may be used to detect AFB1 in food and has potential for the development of test kits.

中文翻译：

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用于测定花生中黄曲霉毒素 B1 的新型酶联适体分析

摘要 报道了一种用于测定黄曲霉毒素 B1 (AFB1) 的新型酶联适体测定法。AFB1 可以与固定化的生物素-适配体竞争性结合并释放生物素互补 DNA，导致检测系统颜色随着 AFB1 浓度的增加而逐渐褪色。在没有 AFB1 的情况下，生物素化的互补 DNA 不会从固定的适体中释放出来。因此，酶反应发生在检测系统中。在优化的实验条件下，所提出的方法对 AFB1 具有从 1 到 80 ng/mL 的宽线性范围（R2 为 0.990），检测限低至 0.36 ng/mL。然后将该方法应用于检测添加不同浓度 AFB1 的未受污染花生。回收率从 82.60% 到 94.43%，