Chemiluminescent western blot (WB) is often performed sequentially for detection of overlapping proteins; in between, prior antibodies must be stripped or the conjugated horseradish peroxidase (HRP) inactivated. However, often, stripping either is insufficient to remove all the bound antibodies or causes protein loss, whereas treatment with hydrogen peroxide, a popular way to inactivate HRP, may affect epitope recognition as the authors previously reported. To date, an ideal method for sequential chemiluminescent WB is still missing. Here it is demonstrated that acid equivalent to 10% acetic acid can efficiently inactivate HRP, allowing sequential probing without protein loss or epitope damage.

中文翻译：

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酸灭活辣根过氧化物酶以进行顺序化学发光Western印迹。

化学发光蛋白质印迹法（WB）通常是顺序进行的，以检测重叠的蛋白；在这两者之间，必须先去除先前的抗体，或者将偶联的辣根过氧化物酶（HRP）灭活。但是，通常剥脱作用不足以去除所有结合的抗体或引起蛋白质损失，而正如过往作者所报道的那样，过氧化氢处理是一种常见的使HRP失活的方法，可能会影响表位的识别。迄今为止，仍然缺少用于连续化学发光WB的理想方法。在此证明，相当于10％乙酸的酸可以有效地使HRP失活，从而可以进行连续的探查，而不会丢失蛋白质或破坏表位。