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Cascaded DNA circuits-programmed self-assembly of spherical nucleic acids for high signal amplification
Science China Chemistry ( IF 9.6 ) Pub Date : 2019-10-21 , DOI: 10.1007/s11426-019-9603-3
Xiang Li , Dongbao Yao , Junxiang Zhou , Xiang Zhou , Xianbao Sun , Bing Wei , Chengxu Li , Bin Zheng , Haojun Liang

Signal amplification is an important issue in DNA nanotechnology and molecular diagnostics. In this work, we report a strategy for the catalytic self-assembly of spherical nucleic acids (SNAs) programmed by two-layer cascaded DNA circuits through integrating an entropy-driven catalytic network, a catalytic hairpin assembly circuit, and a facile SNA assembly-based reporter system. This integrated system could implement ~100,000-fold signal amplification in the presence of 1 pM of input target. Possessing powerful amplification ability of nucleic acid signal, our strategy should be of great potential in fabricating more robust dynamic networks to be applied for signal transduction, DNA computing, and nucleic acid-based diagnostics.

中文翻译:

级联的DNA电路-程序设计的球形核酸自组装以实现高信号放大

信号放大是DNA纳米技术和分子诊断中的重要问题。在这项工作中,我们报告了通过整合熵驱动的催化网络,催化发夹组装电路和简便的SNA组装,由两层级联DNA电路编程的球形核酸(SNA)催化自组装策略。基于记者系统。该集成系统可以在1 pM输入目标存在的情况下实现约100,000倍的信号放大。拥有强大的核酸信号放大能力,我们的策略在制造更强大的动态网络以用于信号转导,DNA计算和基于核酸的诊断中应该具有巨大的潜力。
更新日期:2019-10-23
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