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A Universal Platform for High‐Efficiency “Engineering” Living Cells: Integration of Cell Capture, Intracellular Delivery of Biomolecules, and Cell Harvesting Functions
Advanced Functional Materials ( IF 19.0 ) Pub Date : 2019-10-22 , DOI: 10.1002/adfm.201906362
Yangcui Qu 1 , Yanjun Zheng 1 , Liyin Yu 1 , Yang Zhou 1 , Yaran Wang 1 , Yanxia Zhang 2 , Qian Yu 1 , Hong Chen 1
Affiliation  

A universal platform for the efficient intracellular delivery of biomacromolecules with minimal trauma to the cells is highly desirable for biological research and clinical applications. Moreover, such a platform should include the ability to harvest the “engineered” cells, for particular in vitro or ex vivo conditions. Herein, a broadly applicable platform is presented with integrated multifunctions based on silicon nanowire arrays (SiNWAs) modified with a sugar‐responsive polymer containing phenylboronic acid (PBA) groups. Due to the synergistic effects of the specific recognition of PBA groups by sialic acid and “nanoenhancement” by the SiNWAs, this system shows a high capture capacity for both surface adherent and suspension cells overexpressing sialic acid on the membrane. Under appropriate near‐infrared irradiation, the photothermal properties of SiNWAs endow this system with high efficiency to deliver biomacromolecules into the captured cells by a membrane disruption mechanism. The cells thus “engineered” can be harvested simply by treatment with a nontoxic sugar solution, thereby maintaining good viability for subsequent applications. This method appears to have strong potential for the intracellular delivery of diverse biomacromolecules into both surface adherent and suspension cells, including hard‐to‐transfect suspension T cells, and may open up new pathways for engineering living cells.

中文翻译:

高效“工程化”活细胞的通用平台:细胞捕获,生物分子的细胞内递送和细胞采集功能的整合

对于生物学研究和临床应用而言,非常需要一种通用的平台来有效地细胞内递送生物大分子而对细胞的损伤最小。此外,这种平台应具有收集“工程化”细胞的能力,特别是在体外或离体条件下。在此,展示了一个广泛适用的平台,该平台具有基于硅纳米线阵列(SiNWA)的集成多功能,该纳米线被含苯硼酸(PBA)基团的糖响应性聚合物修饰。由于唾液酸对PBA基团的特异性识别和SiNWA的“纳米增强”的协同作用,该系统对膜上过表达唾液酸的表面粘附细胞和悬浮细胞均显示出很高的捕获能力。在适当的近红外辐射下,SiNWA的光热特性赋予该系统以高效率,可通过膜破坏机制将生物大分子传递到捕获的细胞中。如此“工程化”的细胞可以简单地通过用无毒糖溶液处理而收获,从而为后续应用保持良好的生存能力。这种方法对于将多种生物大分子细胞内递送至表面粘附细胞和悬浮细胞(包括难以转染的悬浮T细胞)具有强大的潜力,并可能为工程化活细胞开辟新途径。从而为后续应用保持良好的生存能力。这种方法对于将多种生物大分子细胞内递送至表面粘附细胞和悬浮细胞(包括难以转染的悬浮T细胞)具有强大的潜力,并可能为工程化活细胞开辟新途径。从而为后续应用保持良好的生存能力。这种方法对于将多种生物大分子细胞内递送至表面粘附细胞和悬浮细胞(包括难以转染的悬浮T细胞)具有强大的潜力,并可能为工程化活细胞开辟新途径。
更新日期:2020-01-17
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