The ability of Saccharomyces cerevisiae to reconstitute viability after strong peroxide-induced oxidative stress during liquid holding (LH) in non-nutrient medium has been compared with that of Ustilago maydis. It was found that like U. maydis, S. cerevisiae was capable of reconstituting viability through multiplication of the survivors. However, differences were observed in the pattern of their response: (i) the reconstitution of viability was slower in S. cerevisiae; (ii) before the viability was progressively increasing the treated samples of this fungus reproducibly passed through a phase of additional decrease of the surviving fraction and (iii) the final yields of viable cells attained in S. cerevisiae were below those achieved by U. maydis. The reason for the relative superiority of U. maydis is twofold: (1) early initiated and faster degradation and leakage of the intracellular biomolecules and (2) greater ability of U. maydis cells to recycle damaged and released intracellular compounds. Conceptually similar studies extended to another oxidative-stress-inducing condition, namely desiccation, indicated that the marked differences between these fungi in their patterns of the post-stress regrowth, cellular leakage and macromolecule decomposition are reproduced during LH of desiccated cells. The concordance of the findings obtained upon these two approaches was also corroborated by an analysis of the post-desiccation LH response of U. maydis mutants (adr1, did4, kel1 and tbp1) that were previously identified as defective in post-peroxide LH restitution of viability. We discuss the findings in terms of their possible relevance to the mechanisms of the ecological and evolutionary adaptation of free-living microorganisms to fluctuating and severely inhospitable environments.

中文翻译：

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玉米Us和酿酒酵母细胞群体中氧化应激后液体保持力恢复活力的比较研究。

比较了酿酒酵母在非营养培养基中持液（LH）期间强过氧化物诱导的氧化应激后恢复活力的能力。发现与酿酒酵母一样，酿酒酵母也能够通过幸存者的繁殖来重建生存力。但是，在反应方式上观察到差异：（i）酿酒酵母的活力重建较慢；（ii）在存活力逐渐增加之前，该真菌的处理样品可重复通过存活分数的进一步降低阶段；（iii）在酿酒酵母中获得的活细胞的最终产量低于梅迪酵母所获得的最终产量。 。U. maydis相对优势的原因有两个：（1）细胞内生物分子的早期启动和更快的降解与渗漏，以及（2）Maydis U. maydis细胞具有更大的能力回收受损和释放的细胞内化合物。概念上相似的研究扩展到了另一种氧化应激诱导条件，即干燥，表明在干燥细胞的LH过程中，这些真菌在应激后的再生，细胞渗漏和大分子分解方面表现出明显差异。通过分析先前被鉴定为过氧化氢的LH还原缺陷的U. maydis突变体（adr1，did4，kel1和tbp1）的干燥后LH反应，也证实了通过这两种方法获得的结果的一致性。可行性。