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Alleviating chronic kidney disease progression through modulating the critical genus of gut microbiota in a cisplatin-induced Lanyu pig model
Journal of Food and Drug Analysis ( IF 3.6 ) Pub Date : 2020-01-01 , DOI: 10.1016/j.jfda.2019.10.001
Ya-Jane Lee , Kuan-Yi Li , Pin-Jhu Wang , Hsiao-Wen Huang , Ming-Ju Chen

In the present study, we investigated the effects of Probiotic mix 1 (Pm1) with Lactobacillus plantarum subsp. plantarum, Lactobacillusparacasei subsp. paracasei, and Streptococcus salivarius subsp. thermophilus on preventing renal injury using a chronic kidney disease (CKD) minipig model previously developed in our lab using cisplatin-induced CKD in Lanyu pigs. The results indicated that the high dosage Pm1 (H.Pm1) group demonstrated lower incidence of lesions, including atrophy, mononuclear inflammation, cell infiltration, and interstitial fibrosis in renal tubules in hematoxylin and eosin (H&E) and Masson's trichrome stain. We further systematically investigated the preventing effect of Pm1. The H.Pm1 group decreased inflammatory cytokines production and increased the level of superoxide dismutase activity in plasma. The pigs fed with high dosage of Pm1 group also showed reduced both creatinine and blood urea nitrogen (BUN) when compared with the cisplatin group. Microbiota results indicated that Pm1-intervention not only reduced the abundance of Gram-negative bacteria but also affected the abundance of specific genera biomarkers, Anaerovibrio, possible_genus_SK018, Holdemanella, and Lachnospiraceae_UCG_010 in gut microbiota, leading to decreased inflammation and apoptosis in the kidney and further prevention/alleviation of the symptoms of CKD.

中文翻译:

在顺铂诱导的蓝鱼猪模型中通过调节肠道微生物群的关键属来缓解慢性肾病进展

在本研究中,我们研究了益生菌混合物 1 (Pm1) 与植物乳杆菌亚种的影响。植物乳杆菌,副干酪乳杆菌亚种。副干酪和唾液链球菌亚种。嗜热菌使用慢性肾病 (CKD) 小型猪模型预防肾损伤,该模型之前在我们实验室中使用顺铂诱导的 CKD 在兰玉猪中开发。结果表明,高剂量 Pm1 (H.Pm1) 组在苏木精和伊红 (H&E) 和马森三色染色中表现出较低的病变发生率,包括萎缩、单核炎症、细胞浸润和肾小管间质纤维化。我们进一步系统地研究了 Pm1 的预防作用。H.Pm1 组减少了炎症细胞因子的产生并增加了血浆中超氧化物歧化酶的活性水平。与顺铂组相比,饲喂高剂量 Pm1 组的猪也表现出肌酐和血尿素氮 (BUN) 降低。微生物群结果表明,Pm1 干预不仅降低了革兰氏阴性菌的丰度,而且影响了肠道微生物群中特定属生物标志物 Anaerovibrio、 possible_genus_SK018、Holdemanella 和 Lachnospiraceae_UCG_010 的丰度,导致肾脏炎症和细胞凋亡减少,并进一步预防/缓解 CKD 的症状。
更新日期:2020-01-01
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