The repeatability of dynamic proteome profiling (DPP), which is a novel technique for measuring the relative abundance (ABD) and fractional synthesis rate (FSR) of proteins in humans, is investigated. LC-MS analysis is performed on muscle samples taken from male participants (n = 4) that consumed 4 × 50 mL doses of deuterium oxide (2 H2 O) per day for 14 days. ABD is measured by label-free quantitation and FSR is calculated from time-dependent changes in peptide mass isotopomer abundances. One-hundred one proteins have at least one unique peptide and are used in the assessment of protein ABD. Fifty-four of these proteins meet more stringent criteria and are used in the assessment of FSR data. The median (M), lower-, (Q1 ) and upper-quartile (Q3 ) values for protein FSR (%/d) are M = 1.63, Q1 = 1.07, and Q3 = 3.24, respectively. The technical CV of ABD data has a median value of 3.6% (Q1 1.7% to Q3 6.7%), whereas the median CV of FSR data is 10.1% (Q1 3.5% to Q3 16.5%). These values compare favorably against other assessments of technical repeatability of proteomics data, which often set a CV of 20% as the upper bound of acceptability.

中文翻译：

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人骨骼肌中蛋白质丰度的可靠性和合成测量。

研究了动态蛋白质组图谱（DPP）的可重复性，这是一种用于测量人体蛋白质的相对丰度（ABD）和分数合成速率（FSR）的新技术。LC-MS分析是从男性参与者（n = 4）采集的肌肉样本上进行的，该样本每天消耗4×50 mL氘化氢（2 H2 O），持续14天。ABD通过无标记定量测量，FSR由肽质量同位素异构体丰度随时间的变化计算得出。一百一种蛋白质具有至少一种独特的肽，并用于评估蛋白质ABD。这些蛋白质中有54种符合更严格的标准，并用于FSR数据评估。蛋白质FSR的中位数（M），下位数（Q1）和上四分位数（Q3）（％/ d）分别为M = 1.63，Q1 = 1.07和Q3 = 3.24。ABD数据的技术CV中位数为3.6％（Q1为1.7％至Q3 6.7％），而FSR数据的CV中位数为10.1％（Q1 3.5％至Q3 16.5％）。这些值与蛋白质组学数据的技术可重复性的其他评估相比具有优势，后者通常将CV设置为20％作为可接受性的上限。