Cetylpyridinium chloride (CPC) is a quaternary ammonium sanitizer approved for fresh poultry animal carcass sanitization from microbial human pathogens, such as Salmonella enterica. Nonetheless, the interactions of CPC with Salmonella cells, and the mechanism of the sanitizer’s neutralization by lecithin remains largely unknown. This study aimed to investigate the interaction of CPC with lecithin and Salmonella Typhimurium to determine the interactions of the sanitizer and neutralizer impacting the bacterium’s survival. Application of 0.8% CPC is proposed to produce loss of microbial membrane integrity with loss of electrostatic repulsion between individual cells, resulting in the eventual emulsification of membrane lipids with cytoplasmic contents leakage. Our findings point to a two-phase interaction between CPC and lecithin impacting S. Typhimurium survival. The first consists of electrostatic attraction and charge neutralization between oppositely charged components of pathogen cell and CPC. The second involves formation of aggregates between sanitizer and pathogen, or between sanitizer, pathogen membrane lipids, and lecithin.

氯化十六烷基吡啶鎓（CPC）是一种季铵消毒剂，已批准用于从微生物人类病原体（如小肠沙门氏菌）消毒新鲜的家禽动物尸体。尽管如此，CPC与沙门氏菌细胞之间的相互作用以及卵磷脂对消毒剂的中和机理仍然未知。这项研究旨在调查CPC与卵磷脂和沙门氏菌的相互作用鼠伤寒以确定消毒剂和中和剂的相互作用影响细菌的存活。建议使用0.8％的CPC来造成微生物膜完整性的丧失以及单个细胞之间静电排斥的丧失，从而最终导致膜脂质的乳化和细胞质内容物的泄漏。我们的发现指向CPC和卵磷脂影响S的两阶段相互作用。鼠伤寒生存。第一个包括病原体细胞和CPC的带相反电荷的成分之间的静电吸引和电荷中和。第二个涉及在消毒剂和病原体之间或在消毒剂，病原体膜脂质和卵磷脂之间形成聚集体。