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Spontaneous mutation in the AgrRS two-component regulatory system of Cupriavidus metallidurans results in enhanced silver resistance.
Metallomics ( IF 3.4 ) Pub Date : 2019-10-14 , DOI: 10.1039/c9mt00123a
Kristel Mijnendonckx 1 , Md Muntasir Ali 2 , Ann Provoost 1 , Paul Janssen 1 , Max Mergeay 1 , Natalie Leys 1 , Daniël Charlier 3 , Pieter Monsieurs 1 , Rob Van Houdt 1
Affiliation  

The uncontrolled and widespread use of (nano)silver compounds has led to the increased release of these compounds into the environment, raising concerns about their negative impact on ecosystems. Concomitantly, silver resistance determinants are widely spread among environmental and clinically relevant bacteria although the underlying mechanisms are not yet fully understood. We show that Cupriavidus metallidurans is able to adapt to toxic silver concentrations. However, none of the known silver resistance determinants present in C. metallidurans are involved in the adaptative response. Instead, increased silver resistance is achieved by the concerted action of a two-component system AgrR–AgrS, previously not associated with metal resistance, and two periplasmic proteins PrsQ1 and PrsQ2. Both proteins belong to an unique group of small, uncharacterized, secreted proteins restricted to the genera Cupriavidus and Ralstonia. This system gives C. metallidurans the ability to withstand much higher silver concentrations. The latter could be facilitated by the accumulation of silver ions and the formation of silver nanoparticles.

中文翻译:

铜绿铜霉的AgrRS两组分调节系统中的自发突变导致银抗性增强。

(纳米)银化合物的不受控制和广泛使用已导致这些化合物向环境中的释放增加,引起人们对它们对生态系统的负面影响的担忧。同时,尽管尚不完全了解其潜在机制,但银抗性决定簇广泛分布于环境和临床相关细菌中。我们表明,Cupriavidus metallidurans能够适应有毒的银浓度。但是,在金属利尿果球菌中存在的任何已知的银抗性决定因素均不涉及适应性反应。相反,通过以前不与金属抗性相关的两组分系统AgrR–AgrS和两个周质蛋白PrsQ的协同作用,可以提高银的抗性。1和PrsQ 2。两种蛋白质都属于一个独特的小,未表征的分泌蛋白质组,这些蛋白质仅限于CupriavidusRalstonia属。该系统使金属里氏梭菌能够承受更高的银浓度。后者可以通过银离子的积累和银纳米颗粒的形成来促进。
更新日期:2019-10-14
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