Lake trout are used as bioindicators for toxics exposure in the Great Lakes ecosystem. Here the first lake trout (Salvelinus namaycush) liver proteomics study is performed and searched against specific databases: (NCBI and UniProtKB) Salvelinus, Salmonidae, Actinopterygii, and Oncorhynchus mykiss, and the more distant relative, Danio rerio. In the biological replicate 1 (BR1), technical replicate 1 (TR1), (BR1TR1), a large number of lake trout liver proteins are not in the Salvelinus protein database, suggesting that lake trout liver proteins have homology to some proteins from the Salmonidae family and Actinopterygii class, and to Oncorhynchus mykiss and Danio rerio, two more highly studied fish. In the NCBI search, 4194 proteins are identified: 3069 proteins in Actinopterygii, 1617 in Salmonidae, 68 in Salvelinus, 568 in Oncorhynchus mykiss, and 946 in Danio rerio protein databases. Similar results are observed in the UniProtKB searches of BR1RT1, as well as in a technical replicate (BR1TR2), and then in a second biological replicate experiment, with two technical replicates (BR2TR1 and BR2TR2). This study opens the possibility of identifying evolutionary relationships (i.e., adaptive mutations) between various groups (i.e., zebrafish, rainbow trout, Salmonidae, Salvelinus and lake trout) through evolutionary proteomics. Data are available via the PRIDE Q2 (PXD011924).

中文翻译：

---

鳟鱼（Salvelinus namaycush）肝脏的蛋白质组学分析可鉴定出进化密切和远缘鱼类亲戚中的蛋白质。

鳟鱼被用作大湖生态系统中有毒物质暴露的生物指标。在这里，进行了第一个湖鳟（Salvelinus namaycush）肝蛋白质组学研究，并针对以下特定数据库进行了搜索：（NCBI和UniProtKB）Salvelinus，Salmonidae，Actinopterygii和Oncorhynchus mykiss，以及更远的亲戚Danio rerio。在生物学复制品1（BR1），技术复制品1（TR1），（BR1TR1）中，鲑鱼鳟鱼肝蛋白不在Salvelinus蛋白质数据库中，这表明鲑鱼鳟鱼肝蛋白与鲑科的某些蛋白具有同源性家族和放线藻类，以及对Oncorhynchus mykiss和Danio rerio的研究更多的两条鱼。在NCBI搜索中，鉴定出4194种蛋白质：放线菌中的3069种蛋白质，鲑科中的1617种，鲑鱼中的68种，Mycorhynchus mykiss中的568种，和946在Danio rerio蛋白质数据库中。在UniProtKB搜索BR1RT1和技术复制品（BR1TR2）中，然后在第二次生物学复制实验中，使用两个技术复制品（BR2TR1和BR2TR2），也观察到了相似的结果。这项研究为通过进化蛋白质组学鉴定各种群体（斑马鱼，虹鳟，鲑科，鲑鱼和湖鳟）之间的进化关系（即适应性突变）提供了可能性。数据可通过PRIDE Q2（PXD011924）获得。这项研究为通过进化蛋白质组学鉴定各种群体（斑马鱼，虹鳟，鲑科，鲑鱼和湖鳟）之间的进化关系（即适应性突变）提供了可能性。数据可通过PRIDE Q2（PXD011924）获得。这项研究为通过进化蛋白质组学鉴定各种群体（斑马鱼，虹鳟，鲑科，鲑鱼和湖鳟）之间的进化关系（即适应性突变）提供了可能性。数据可通过PRIDE Q2（PXD011924）获得。