A new polyclonal antibody that recognizes the CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4-EPSPS), which provides resistance to glyphosate in soybean (Roundup Ready®, RR soybean), was produced. New Zealand rabbits were injected with a synthetic peptide (Pc_312-324, (PEP)) present in the soybean CP4-EPSPS protein. The anti-PEP antibodies production was evaluated by electrophoresis (SDS-PAGE) and an enzyme-linked immunosorbent assay (ELISA) was developed in order to study their specificity. The ELISA showed that the polyclonal antibody was specific to PEP. In addition, the anti- PEP was immobilized onto a gold disk electrode and the antigen-antibody interaction was evaluated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Moreover, the EIS showed that the electron transfer resistance of the modified electrode increased after incubation with solutions containing CP4-EPSPS protein from RR transgenic soybean, while no changes were detected after incubation with no-RR soybean proteins. These results suggest that the CP4-EPSPS was immobilized onto the electrode, due to the specific interaction with the anti-PEP. These results show that this antigen-antibody interaction can be detected by electrochemical techniques, suggesting that the anti-PEP produced can be used in electrochemical immunosensors development to quantify transgenic soybean.

中文翻译：

---

多克隆抗体产生抗Pc_312-324肽。它在电化学免疫传感器中用于转基因大豆检测的潜在用途。

产生了一种新的多克隆抗体，该抗体可识别CP4 5-烯丙基丙酮酸shi草酸酯-3-磷酸合酶（CP4-EPSPS），该抗体可抵抗大豆（RoundupReady®，RR大豆）中的草甘膦。新西兰兔子注射了大豆CP4-EPSPS蛋白中存在的合成肽（Pc_312-324，（PEP））。通过电泳（SDS-PAGE）评估了抗PEP抗体的产生，并开发了一种酶联免疫吸附测定（ELISA）来研究其特异性。ELISA显示多克隆抗体对PEP具有特异性。此外，将抗PEP固定在金盘电极上，并使用循环伏安法（CV）和电化学阻抗谱（EIS）评估抗原-抗体相互作用。而且，EIS表明，与含有RR转基因大豆的CP4-EPSPS蛋白的溶液一起孵育后，修饰电极的电子传递阻力增加，而与无RR大豆蛋白一起孵育后未发现变化。这些结果表明，由于与抗PEP的特异性相互作用，CP4-EPSPS被固定在电极上。这些结果表明该抗原-抗体相互作用可以通过电化学技术检测，表明产生的抗PEP可用于电化学免疫传感器开发中以定量转基因大豆。这些结果表明，由于与抗PEP的特异性相互作用，CP4-EPSPS被固定在电极上。这些结果表明该抗原-抗体相互作用可以通过电化学技术检测，表明产生的抗PEP可用于电化学免疫传感器开发中以定量转基因大豆。这些结果表明，由于与抗PEP的特异性相互作用，CP4-EPSPS被固定在电极上。这些结果表明该抗原-抗体相互作用可以通过电化学技术检测，表明产生的抗PEP可用于电化学免疫传感器开发中以定量转基因大豆。