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Lipoic acid antagonizes paraquat-induced vascular endothelial dysfunction by suppressing mitochondrial reactive oxidative stress.
Toxicology Research ( IF 2.1 ) Pub Date : 2019-09-27 , DOI: 10.1039/c9tx00186g
Li Pang 1 , Ping Deng 2 , Yi-Dan Liang 1 , Jing-Yu Qian 1 , Li-Chuan Wu 1 , Ling-Ling Yang 2 , Zheng-Ping Yu 1, 2 , Zhou Zhou 1
Affiliation  

Paraquat (PQ) is a widely used herbicide in the agricultural field. The lack of an effective antidote is the significant cause of high mortality in PQ poisoning. Here, we investigate the antagonistic effects of alpha lipoic acid (α-LA), a naturally existing antioxidant, on PQ toxicity in human microvascular endothelial cells (HMEC-1). All the doses of 250, 500 and 1000 μM α-LA significantly inhibited 1000 μM PQ-induced cytotoxicity in HMEC-1 cells. α-LA pretreatment remarkably diminished the damage to cell migration ability, recovered the declined levels of the vasodilator factor nitric oxide (NO), elevated the expression level of endothelial nitric oxide synthases (eNOS), and inhibited the upregulated expression of vasoconstrictor factor endothelin-1 (ET-1). Moreover, α-LA pretreatment inhibited reactive oxygen species (ROS) generation, suppressed the damage to the mitochondrial membrane potential (ΔΨm) and mitigated the inhibition of adenosine triphosphate (ATP) production in HMEC-1 cells. These results suggested that α-LA could alleviate PQ-induced endothelial dysfunction by suppressing oxidative stress. In summary, our present study provides novel insight into the protective effects and pharmacological potential of α-LA against PQ toxicity in microvascular endothelial cells.

中文翻译:

硫辛酸通过抑制线粒体反应性氧化应激,拮抗百草枯引起的血管内皮功能障碍。

百草枯(PQ)是农业领域中广泛使用的除草剂。缺乏有效的解毒剂是PQ中毒高死亡率的重要原因。在这里,我们研究了自然存在的抗氧化剂α硫辛酸(α-LA)对人微血管内皮细胞(HMEC-1)中PQ毒性的拮抗作用。250、500和1000μMα-LA的所有剂量均可显着抑制HMEC-1细胞中1000μMPQ诱导的细胞毒性。α-LA预处理显着减少了对细胞迁移能力的损害,恢复了血管扩张因子一氧化氮(NO)下降的水平,升高了内皮一氧化氮合酶(eNOS)的表达水平,并抑制了血管收缩因子内皮素的上调表达1(ET-1)。此外,α-LA预处理可抑制活性氧(ROS)的产生,Ψ)和减轻三磷酸腺苷(ATP)生产的HMEC-1细胞的抑制。这些结果表明,α-LA可以通过抑制氧化应激减轻PQ诱导的内皮功能障碍。总而言之,我们的研究为α-LA对微血管内皮细胞中PQ毒性的保护作用和药理潜力提供了新的见解。
更新日期:2019-09-27
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