The metabolic enzyme CTP synthase (CTPS) is able to compartmentalize into filaments, termed cytoophidia, in a variety of organisms including bacteria, budding yeast, fission yeast, fruit flies and mammals. A previous study in budding yeast shows that the filament-forming process of CTPS is not sensitive to temperature shift. Here we study CTPS filamentation in the fission yeast Schizosaccharomyces pombe. To our surprise, we find that both the length and the occurrence of cytoophidia in S. pombe decrease upon cold shock or heat shock. The temperature-dependent changes of cytoophidia are fast and reversible. Taking advantage of yeast genetics, we demonstrate that heat-shock proteins are required for cytoophidium assembly in S. pombe. Temperature sensitivity of cytoophidia makes S. pombe an attractive model system for future investigations of this novel membraneless organelle.

代谢酶CTP合酶（CTPS）能够在包括细菌，出芽酵母，裂变酵母，果蝇和哺乳动物在内的各种生物体中分隔成细丝，称为胞吞。先前对发芽酵母的研究表明，CTPS的长丝形成过程对温度变化不敏感。在这里我们研究裂殖酵母裂殖酵母中CTPS的丝状化。令我们惊讶的是，我们发现在冷休克或热休克后，粟酒裂殖酵母的长度和胞吞现象都减少了。温度依赖的细胞吞噬变化是快速且可逆的。利用酵母遗传学，我们证明了热休克蛋白是粟酒裂殖酵母中细胞膜组装所必需的。嗜细胞的温度敏感性使粟酒裂殖酵母成为该新型无膜细胞器未来研究的诱人模型系统。