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Colonization and immune modulation properties of Klebsiella pneumoniae biofilm-dispersed cells.
npj Biofilms and Microbiomes ( IF 9.2 ) Pub Date : 2019-09-24 , DOI: 10.1038/s41522-019-0098-1
Cyril Guilhen , Sylvie Miquel , Nicolas Charbonnel , Laura Joseph , Guillaume Carrier , Christiane Forestier , Damien Balestrino

Biofilm-dispersal is a key determinant for further dissemination of biofilm-embedded bacteria. Recent evidence indicates that biofilm-dispersed bacteria have transcriptional features different from those of both biofilm and planktonic bacteria. In this study, the in vitro and in vivo phenotypic properties of Klebsiella pneumoniae cells spontaneously dispersed from biofilm were compared with those of planktonic and sessile cells. Biofilm-dispersed cells, whose growth rate was the same as that of exponential planktonic bacteria but significantly higher than those of sessile and stationary planktonic forms, colonized both abiotic and biotic surfaces more efficiently than their planktonic counterparts regardless of their initial adhesion capabilities. Microscopy studies suggested that dispersed bacteria initiate formation of microcolonies more rapidly than planktonic bacteria. In addition, dispersed cells have both a higher engulfment rate and better survival/multiplication inside macrophages than planktonic cells and sessile cells. In an in vivo murine pneumonia model, the bacterial load in mice lungs infected with biofilm-dispersed bacteria was similar at 6, 24 and 48 h after infection to that of mice lungs infected with planktonic or sessile bacteria. However, biofilm-dispersed and sessile bacteria trend to elicit innate immune response in lungs to a lesser extent than planktonic bacteria. Collectively, the findings from this study suggest that the greater ability of K. pneumoniae biofilm-dispersed cells to efficiently achieve surface colonization and to subvert the host immune response confers them substantial advantages in the first steps of the infection process over planktonic bacteria.



中文翻译:

肺炎克雷伯菌生物膜分散细胞的定殖和免疫调节特性。

生物膜分散是进一步传播嵌入生物膜的细菌的关键决定因素。最近的证据表明,生物膜分散细菌的转录特征不同于生物膜细菌和浮游细菌。在这项研究中,肺炎克雷伯菌的体外和体内表型特性将自生物膜自发分散的细胞与浮游和无柄细胞进行比较。生物膜分散细胞的生长速度与指数型浮游细菌的生长速度相同,但明显高于无柄和固定浮游形式的细胞,无论其初始粘附能力如何,其生物和生物表面都比其浮游生物更有效地定殖。显微镜研究表明,分散的细菌比浮游细菌更快地开始形成微生物菌落。另外,与浮游细胞和无柄细胞相比,分散的细胞在巨噬细胞内部具有更高的吞噬率和更好的存活/繁殖。在体内鼠肺炎模型中,感染生物膜分散细菌的小鼠肺部细菌载量在6岁时相似。感染后24和48小时,小鼠的肺部感染了浮游细菌或无柄细菌。然而,与浮游细菌相比,分散在生物膜上的无柄细菌倾向于在肺中引发先天免疫反应。总体而言,这项研究的结果表明,肺炎克雷伯菌的生物膜分散细胞可以有效地实现表面定殖并破坏宿主的免疫反应,从而在感染过程的第一步中具有比浮游细菌更大的优势。

更新日期:2019-09-24
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