This study aimed to analyze spatial patterns of soil ectomycorrhizal fungal (EMF) communities at the local scale in a subtropical pine-oak forest located in the Nearctic-Neotropical transition in central Mexico, to underpin biodiversity conservation strategies in forest fragments of this region. We used a spatially-explicit nested square sampling design with the same sampling representativeness at all scales and replicated three times. We detected 674 EMF OTUs within 19,200 m² and 65 OTUs on average per sample. Seventy percent of OTUs were detected in only 1–4 samples. Average community similarity was below 5%, showed minor change within 14 and 339 m distance and increased with the spatial grain used to compare the data. We found a high species-area relationship and beta diversity coefficients for soil fungi indicating that, at the local scale, increasing area by a constant factor of four represented an increase in OTU richness by a factor of two.

这项研究旨在分析位于墨西哥中部近热带向新热带过渡的亚热带松栎森林在当地尺度上的土壤外生菌根真菌（EMF）群落的空间格局，以支持该地区森林碎片的生物多样性保护策略。我们使用了空间明晰的嵌套方抽样设计，在所有尺度上均具有相同的抽样代表性，并重复了3次。我们在19,200 m ²内检测到674个EMF OTU每个样本平均65个OTU。仅1-4个样本中检测到70％的OTU。平均群落相似度低于5％，在14和339 m距离内显示较小变化，并随用于比较数据的空间粒度而增加。我们发现土壤真菌具有较高的物种面积关系和β多样性系数，表明在本地范围内，将面积增加4的常数表示OTU丰富度增加了2。