An enzyme degassing method for oxygen-intolerant polymerizations was implemented in a commercially available automated parallel synthesizer and tested for reversible addition-fragmentation chain transfer (RAFT) polymerizations performed in open vessels. For this purpose, a recently reported methodology that employs the enzyme glucose oxidase (GOx) to deplete oxygen in reaction media was utilized. The effectiveness of this approach to perform unattended parallel polymerization reactions in open vessels was demonstrated by comparing experimental results to those obtained under similar experimental conditions but utilizing the common degassing method of sparging N2 to remove oxygen. The proposed experimental technique displayed good precision in performing RAFT polymerizations and good control of the obtained polymers and could be easily adapted to other systems where the removal of oxygen is mandatory. This alternative high-throughput/high-output method may have the potential to increase productivity in research projects where oxygen-intolerant reactions are involved.

中文翻译：

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自动并行合成器开放容器中的氧气敏感反应的酶脱气：RAFT聚合。

用于氧不耐受聚合的酶脱气方法是在可商购的自动并行合成器中实施的，并测试了在敞口容器中进行的可逆加成-断裂链转移（RAFT）聚合反应。为了这个目的，使用了最近报道的使用酶葡萄糖氧化酶（GOx）来消耗反应介质中的氧气的方法。通过将实验结果与在类似实验条件下但使用喷射N2的常规脱气方法脱氧的实验结果进行比较，证明了该方法在开放容器中进行无人参与的平行聚合反应的有效性。提出的实验技术在进行RAFT聚合反应中显示出良好的精度，并对得到的聚合物进行了良好的控制，并且可以轻松地适用于其他必须强制除氧的系统。这种高通量/高产量的替代方法可能会提高涉及不耐氧反应的研究项目的生产率。