A tryptophan (Trp) molecularly imprinted electrochemical sensor was fabricated by drop-coating an imprinted chitosan film on the surface of a glassy carbon electrode modified with multi-walled carbon nanotubes (MIP-MWCNTs/GCE). The surface morphology and electrochemical properties of the MIP-MWCNTs/GCE were characterized by scanning electron microscopy (SEM) and cyclic voltammetry (CV), respectively. The formation of hydrogen bonds between the functional polymer and the template molecule was confirmed by infrared spectroscopy. The electrochemical performance of the MIP-MWCNTs/GCE with Trp showed that the signal of the oxidation current of Trp obtained with MIP-MWCNTs/GCE was significantly enhanced relative to that of the uncovered GCE, indicating that the modified electrode can accelerate electron transfer and has strong selectivity for Trp. The experimental conditions were optimized in parallel, and under the optimal conditions, the MIP-MWCNTs/GCE showed a good linear relationship between the Trp oxidation peak current and Trp concentrations in the ranges of 2.0 nM-0.2 μM, 0.2 μM-10 μM and 10 μM-100 μM The limit of detection (LOD) was 1.0 nM (S/N = 3), and the modified electrode had good reproducibility and stability. Finally, the MIP-MWCNTs/GCE was successfully applied to the determination of Trp in the human serum samples.

中文翻译：

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碳纳米管和分子印迹聚合物修饰的玻璃碳电极可快速识别和测定色氨酸。

色氨酸（Trp）分子印迹电化学传感器是通过在多壁碳纳米管（MIP-MWCNTs / GCE）修饰的玻璃碳电极表面上滴涂印迹壳聚糖膜而制成的。MIP-MWCNTs / GCE的表面形貌和电化学性能分别通过扫描电子显微镜（SEM）和循环伏安法（CV）表征。通过红外光谱证实了功能聚合物与模板分子之间氢键的形成。具有Trp的MIP-MWCNTs / GCE的电化学性能表明，与未覆盖的GCE相比，由MIP-MWCNTs / GCE获得的Trp的氧化电流信号显着增强，表明修饰的电极可以促进电子转移和加速。对色氨酸具有很强的选择性。并行优化实验条件，在最佳条件下，MIP-MWCNTs / GCE在2.0 nM-0.2μM，0.2μM-10μM和200 nm的范围内，Trp氧化峰值电流与Trp浓度之间具有良好的线性关系。 10μM-100μM检测极限（LOD）为1.0 nM（S / N = 3），并且修饰的电极具有良好的重现性和稳定性。最终，MIP-MWCNTs / GCE成功应用于人血清样品中Trp的测定。修饰电极具有良好的重现性和稳定性。最终，MIP-MWCNTs / GCE成功应用于人血清样品中Trp的测定。修饰电极具有良好的重现性和稳定性。最终，MIP-MWCNTs / GCE成功应用于人血清样品中Trp的测定。