Proximity based detection methods are invaluable tools in the field of molecular biology, increasing selectivity and allowing for analysis of protein interactions. ProxHCR utilizes pairs of antibodies labelled with oligonucleotides to probe for proximal binding and to initiate a hybridization chain reaction (HCR) to generate an amplified detection signal. As HCR is based upon hybridization of DNA hairpins, the performance is dependent on salt concentrations and temperature. Herein we have redesigned the proxHCR system to increase the performance and to reduce dependency on temperature and salt concentrations. The new oligonucleotides provide an increased signal when performed at physiological salt concentrations and in room temperature.

中文翻译：

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优化杂交链反应的邻近依赖性引发，以提高性能

基于邻近的检测方法是分子生物学领域中不可估量的工具，可提高选择性并允许分析蛋白质相互作用。ProxHCR利用标记有寡核苷酸的抗体对探测近端结合并启动杂交链反应（HCR），以产生放大的检测信号。由于HCR基于DNA发夹的杂交，因此性能取决于盐浓度和温度。在此，我们重新设计了proxHCR系统，以提高性能并减少对温度和盐浓度的依赖性。当在生理盐浓度和室温下进行操作时，新的寡核苷酸可提供增强的信号。