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Kaempferol alleviates the reduction of developmental competence during aging of porcine oocytes.
Animal Science Journal ( IF 2 ) Pub Date : 2019-09-04 , DOI: 10.1111/asj.13280
Xuerui Yao 1, 2 , Hao Jiang 2, 3 , Ying-Hua Li 1 , Qingshan Gao 1 , Yong Nan Xu 1 , Nam-Hyung Kim 2
Affiliation  

Kaempferol (KAE) is a natural flavonoid present in different plant species and exhibits anti-inflammatory, antioxidant, and anticancer therapeutic properties. In the present study, we investigated the influence and underlying mechanisms of KAE supplementation on porcine oocytes during in vitro aging. The results show that KAE treatment can alleviate the aging-related reduction of developmental competence. We observed that the blastocyst production rate in aged oocytes treated with 0.1 μM KAE was significantly higher than in untreated aging oocytes (36.78 ± 0.86% vs. 27.55 ± 2.60%, respectively, p < .05). The KAE-treated aging oocytes had significantly reduced levels of reactive oxygen species (p < .05). Furthermore, the mRNA levels of the embryonic pluripotency-related genes Oct4, NANOG, and ITGA5 were significantly increased in blastocysts derived from KAE-treated oocytes (p < .05). During excessive oocyte culture, KAE treatment maintained the mitochondrial membrane potential and reduced apoptosis; however, this was not observed in untreated aging oocytes. In conclusion, our results suggest that KAE treatment can alleviate the aging of porcine oocytes by reducing oxidative stress and improving mitochondrial function.

中文翻译:

山萘酚可减轻猪卵母细胞衰老过程中发育能力的降低。

山emp酚(KAE)是存在于不同植物物种中的天然类黄酮,具有抗炎,抗氧化和抗癌的治疗特性。在本研究中,我们调查了体外老化过程中补充KAE对猪卵母细胞的影响及其潜在机制。结果表明,KAE治疗可以缓解与衰老有关的发育能力下降。我们观察到,用0.1μMKAE处理的老卵母细胞的胚泡产生率显着高于未处理的老卵母细胞(分别为36.78±0.86%和27.55±2.60%,p <.05)。经KAE处理的衰老卵母细胞具有显着降低的活性氧水平(p <.05)。此外,胚胎多能性相关基因Oct4,NANOG,在经KAE处理的卵母细胞中产生的囊胚中,ITA5和ITGA5显着增加(p <.05)。在过度卵母细胞培养过程中,KAE处理可保持线粒体膜电位并减少细胞凋亡。然而,这在未处理的老化卵母细胞中未观察到。总之,我们的结果表明,KAE治疗可以通过降低氧化应激和改善线粒体功能来减轻猪卵母细胞的衰老。
更新日期:2019-09-04
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