The recent introduction of plant-only-based culture media enabled cultivation of not-yet-cultured bacteria that exceed 90% of the plant microbiota communities. Here, we further prove the competence and challenge of such culture media, and further introduce “the inoculum-dependent culturing strategy, IDC”. The strategy depends on direct inoculating plant serial dilutions onto plain water agar plates, allowing bacteria to grow only on the expense of natural nutrients contained in the administered inoculum. Developed colonies are successively transferred/subcultured onto plant-only-based culture media, which contains natural nutrients very much alike to those found in the prepared plant inocula. Because of its simplicity, the method is recommended as a powerful tool in screening programs that require microbial isolation from a large number of diverse plants. Here, the method comfortably and successfully recovered several isolates of endophytic Actinobacteria represented by the six genera of Curtobacterium spp., Plantibacter spp., Agreia spp., Herbiconiux spp., Rhodococcus spp., and Nocardioides spp. Furthermore, two of the isolates are most likely novel species belonging to Agreia spp. and Herbiconiux spp.

最近引入的仅基于植物的培养基能够培养超过90％的植物微生物群落的尚未培养的细菌。在这里，我们进一步证明了这种培养基的能力和挑战，并进一步介绍了“依赖接种物的培养策略，IDC”。该策略取决于将植物系列稀释液直接接种到纯水琼脂平板上，使细菌仅在施用的接种物中包含自然营养成分的情况下才能生长。将发达的菌落相继转移/传代到仅基于植物的培养基中，该培养基包含的天然养分与制备的植物接种物中的养分非常相似。由于其简单性，建议将该方法作为一种强大的工具，用于筛选需要从大量不同植物中分离出微生物的程序。在这里，该方法舒适，成功地回收了由六个属的内生放线菌组成的分离株。Curtobacterium属属，Plantibacter属，Agreia属，Herbiconiux属，红球菌属和诺卡氏菌。此外，两个分离株最有可能属于Agreia spp的新物种。和Herbiconiux spp。