INTRODUCTION Non-targeted metabolic profiling using high-resolution mass spectrometry (HRMS) is a standard approach for pathway identification despite technical limitations. OBJECTIVES To assess the performance of combining targeted quadrupole (QQQ) analysis with HRMS for in-depth pathway profiling. METHODS Serum of exercising patients with type 1 diabetes (T1D) was profiled using targeted and non-targeted assays. RESULTS Non-targeted analysis yielded a broad unbiased metabolic profile, targeted analysis increased coverage of purine metabolism (twofold) and TCA cycle (three metabolites). CONCLUSION Our screening strategy combined the benefits of the unbiased full-scan HRMS acquisition with the deeper insight into specific pathways by large-scale QQQ analysis.

中文翻译：

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通过UHPLC-MS进行的代谢组学：通过将Q-TOF和QQQ互补用于途径分析而提供的好处。

简介尽管有技术限制，但使用高分辨率质谱（HRMS）进行非目标代谢谱分析是途径鉴定的标准方法。目的评估结合靶向四极杆（QQQ）分析与HRMS进行深度路径分析的性能。方法采用有针对性和无针对性的分析方法对1型糖尿病（T1D）运动患者的血清进行分析。结果非目标分析产生了广泛的无偏代谢谱，目标分析增加了嘌呤代谢（两倍）和TCA循环（三种代谢物）的覆盖范围。结论我们的筛选策略将无偏向全扫描HRMS采集的好处与通过大规模QQQ分析对特定途径的更深入了解相结合。