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Efficacy of Active Immunization With Attenuated α-Hemolysin and Panton-Valentine Leukocidin in a Rabbit Model of Staphylococcus aureus Necrotizing Pneumonia.
The Journal of Infectious Diseases ( IF 6.4 ) Pub Date : 2020-01-02 , DOI: 10.1093/infdis/jiz437
Vuvi G Tran 1 , Arundhathi Venkatasubramaniam 2 , Rajan P Adhikari 2 , Subramaniam Krishnan 2 , Xing Wang 1 , Vien T M Le 1 , Hoan N Le 1 , Trang T T Vu 1 , Erika Schneider-Smith 1 , M Javad Aman 2 , Binh An Diep 1
Affiliation  

Staphylococcus aureus is a common pathogen causing infections in humans with various degrees of severity, with pneumonia being one of the most severe infections. In as much as staphylococcal pneumonia is a disease driven in large part by α-hemolysin (Hla) and Panton-Valentine leukocidin (PVL), we evaluated whether active immunization with attenuated forms of Hla (HlaH35L/H48L) alone, PVL components (LukS-PVT28F/K97A/S209A and LukF-PVK102A) alone, or combination of all 3 toxoids could prevent lethal challenge in a rabbit model of necrotizing pneumonia caused by the USA300 community-associated methicillin-resistant S. aureus (MRSA). Rabbits vaccinated with Hla toxoid alone or PVL components alone were only partially protected against lethal pneumonia, whereas those vaccinated with all 3 toxoids had 100% protection against lethality. Vaccine-mediated protection correlated with induction of polyclonal antibody response that neutralized not only α-hemolysin and PVL, but also other related toxins, produced by USA300 and other epidemic MRSA clones.

中文翻译:

减毒的α-溶血素和潘顿-华伦丁白细胞介素在金黄色葡萄球菌坏死性肺炎兔模型中的主动免疫效果。

金黄色葡萄球菌是常见的病原体,其在不同程度的人体内引起感染,其中肺炎是最严重的感染之一。由于葡萄球菌性肺炎在很大程度上是由α-溶血素(Hla)和Panton-Valentine leukocidin(PVL)引起的,我们评估了是否仅使用减毒形式的Hla(HlaH35L / H48L),PVL组分(LukS)主动免疫-PVT28F / K97A / S209A和LukF-PVK102A)的组合,或在3种类毒素的组合中,均可以预防由USA300社区相关耐甲氧西林金黄色葡萄球菌(MRSA)引起的坏死性肺炎兔子模型的致死性攻击。单独接种Hla类毒素或单独使用PVL成分的兔子仅能部分保护其免受致命性肺炎的侵害,而接种所有3种类毒素的兔子具有100%的致死性保护。
更新日期:2019-12-30
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