A newly modified paper-based enzyme-linked immunosorbent assay (P-ELISA) was established by immobilizing more proteins on the paper surface through an atom transfer radical polymerization (ATRP) reaction. In addition, introducing graphene oxide (GO) sheets, Au nanoparticles (AuNps) and two primary antibodies (Ab1s) led to signal amplification and cost reduction.

中文翻译：

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原子转移自由基聚合物改性纸可改善纸基ELISA中蛋白质的固定

通过原子转移自由基聚合（ATRP）反应将更多蛋白质固定在纸张表面，从而建立了一种新的基于纸张的酶联免疫吸附测定法（P-ELISA）。此外，引入氧化石墨烯（GO）片，金纳米颗粒（AuNps）和两种一抗（Ab1s）导致信号放大和成本降低。