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Neutralization of the Positive Charges on Histone Tails by RNA Promotes an Open Chromatin Structure.
Cell Chemical Biology ( IF 8.6 ) Pub Date : 2019-08-22 , DOI: 10.1016/j.chembiol.2019.08.002
Rositsa Dueva 1 , Karen Akopyan 2 , Chiara Pederiva 2 , Davide Trevisan 1 , Soniya Dhanjal 1 , Arne Lindqvist 2 , Marianne Farnebo 3
Affiliation  

RNA associates extensively with chromatin and can influence its structure; however, the potential role of the negative charges of RNA on chromatin structure remains unknown. Here, we demonstrate that RNA prevents precipitation of histones and can attenuate electrostatic interactions between histones and DNA, thereby loosening up the chromatin structure. This effect is independent of the sequence of RNA but dependent on its single-stranded nature, length, concentration, and negative charge. Opening and closure of chromatin by RNA occurs rapidly (within minutes) and passively (in permeabilized cells), in agreement with electrostatics. Accordingly, chromatin compaction following removal of RNA can be prevented by high ionic strength or neutralization of the positively charged histone tails by hyperacetylation. Finally, LINE1 repeat RNAs bind histone H2B and can decondense chromatin. We propose that RNA regulates chromatin opening and closure by neutralizing the positively charged tails of histones, reducing their electrostatic interactions with DNA.

中文翻译:

RNA对组蛋白尾巴上的正电荷的中和促进了染色质的开放结构。

RNA与染色质广泛结合,并可以影响其结构。然而,RNA负电荷对染色质结构的潜在作用仍然未知。在这里,我们证明RNA可以防止组蛋白沉淀,并可以减弱组蛋白和DNA之间的静电相互作用,从而使染色质结构松散。这种作用与RNA序列无关,但取决于其单链性质,长度,浓度和负电荷。RNA与染色质的打开和关闭迅速发生(在数分钟之内),被动发生在渗透性细胞中(与渗透作用一致)。因此,可以通过高离子强度或通过超乙酰化中和带正电荷的组蛋白尾巴来防止RNA去除后的染色质紧缩。最后,LINE1重复RNA结合组蛋白H2B,可以使染色质脱凝。我们建议RNA通过中和组蛋白的带正电荷的尾巴来调节染色质的打开和关闭,减少它们与DNA的静电相互作用。
更新日期:2019-11-09
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