Rational design and synthesis of high-efficiency artificial enzymes with multiple enzyme-like activities in one unit and high selectivity for specific substrates remain great challenges. In this work, for the first time, a stable three-dimensional (3D) Cu-based metal–organic porous network (Cu-MOPN) with intrinsic oxidase- and peroxidase-mimicking activities was successfully fabricated from a one-dimensional (1D) Cu-based metal–organic gel (Cu-MOG) under microwave irradiation. Owing to its high BET surface area, hierarchically porous structure and inherent mass transfer channels, Cu-MOPN shows higher catalytic activity than Cu-MOG. Furthermore, on the basis of the excellent cysteine (Cys) oxidase- and peroxidase-mimicking activities of Cu-MOPN, the highly selective detection of Cys in serum was well realized by a cascade reaction. This study may provide a new strategy for constructing high-efficiency artificial enzymes with versatile functions, and will be highly beneficial for a broad range of applications including biocatalysis, bioassays and biomedicine.

中文翻译：

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尺寸转换：从一维金属有机凝胶到具有高效多重酶样活性的3D金属有机多孔网络，用于级联反应†

在一个单元中具有多种酶样活性和对特定底物的高选择性的高效人工酶的合理设计和合成仍然是巨大的挑战。在这项工作中，首次成功地从一维（1D）制备了具有固有氧化酶和过氧化物酶模拟活性的稳定的三维（3D）铜基金属有机多孔网络（Cu-MOPN）。微波辐射下的铜基金属有机凝胶（Cu-MOG）。由于其较高的BET表面积，分层多孔结构和固有的传质通道，Cu-MOPN的催化活性高于Cu-MOG。此外，基于Cu-MOPN的出色的半胱氨酸（Cys）氧化酶和过氧化物酶模拟活性，通过级联反应很好地实现了对血清中Cys的高选择性检测。