Purification of compounds is a necessary aspect of chemical synthesis. Developing an efficient purification method is time-consuming. A method that quickly calculates preparative gradients from analytical scouting runs is described. A solvent composition that provides the desired retention of a model compound is used to calibrate the analytical scouting run to determine an apparent gradient delay. This delay is applied to the retention times of compounds run with the same scouting gradient to yield a solvent composition for preparative purification.

中文翻译：

---

分析型到制备型液相色谱方法开发概述。

化合物的纯化是化学合成的必要方面。开发有效的纯化方法非常耗时。描述了一种从解析侦查运行快速计算制备梯度的方法。提供所需保留的模型化合物的溶剂组合物用于校准分析筛选操作，以确定表观梯度延迟。该延迟适用于以相同的洗脱梯度运行的化合物的保留时间，以产生用于制备性纯化的溶剂组合物。