Coryneazolicin is a plantazolicin family peptide, belonging to linear azole-containing peptides (LAPs). Although coryneazolicin was previously synthesized by in vitro experiments, its biological activity has not been evaluated. In this report, the heterologous production of coryneazolicin was accomplished to obtain enough coryneazolicin for biological activity tests. The structure of coryneazolicin was confirmed by ESI-MS and NMR analyses. The biological activity tests indicated that coryneazolicin possessed potent antibacterial activity and cytotoxicity. Although antibacterial activity of plantazolicin was previously reported, cytotoxicity was newly found in coryneazolicin among plantazolicin type peptides. In addition, we revealed that coryneazolicin induced apoptosis on HCT116 and HOS cancer cell lines.

中文翻译：

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大肠杆菌中异源生产的炔炔考林。

Coryneazolicin是一种植物唑啉家族肽，属于线性含唑基的肽（LAP）。尽管预先通过体外实验合成了炔炔新星，但尚未对其生物学活性进行评估。在此报告中，完成了异源生产的炔苯并辛酯，以获取足够的炔苯并佐林用于生物活性测试。通过ESI-MS和NMR分析证实了棒炔唑的结构。生物学活性测试表明，Coryneazolicin具有强大的抗菌活性和细胞毒性。尽管先前已经报道了植物唑菌素的抗菌活性，但是在植物唑菌素型肽中的新炔考唑菌素中新发现了细胞毒性。另外，我们揭示了Coryneazolicin诱导了HCT116和HOS癌细胞系的凋亡。