Fucosylated chondroitin sulfates (FCSs) from sea cucumbers have repetitive structures that exhibit minor structural differences based on the organism from which they are recovered. A detailed characterization of FCSs and their derivatives is important to establish their structure–activity relationship in the development of new anticoagulant drugs. In the current study, online hydrophilic interaction chromatography–Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides. These depolymerized FCS fragments were quantified and compared using the glycomics software package, GlycReSoft. The quantified fragments mainly had trisaccharide-repeating compositions and showed significant differences in fucosylation (including its sulfation) among different species of sea cucumbers. Detailed analysis of FTMS ion peaks and top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides verified the accuracy of this method. Thus, a new structural model for FCS chains from these different sea cucumbers was defined. This bottom-up approach provides rich detailed structural analysis and provides quantitative information with high accuracy and reproducibility and should be suitable for the quality control in FCSs as well as their oligosaccharides.

中文翻译：

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使用液相色谱-傅立叶变换质谱自下而上分析表征海参中岩藻糖基化硫酸软骨素

来自海参的岩藻糖基化硫酸软骨素 (FCS) 具有重复结构，根据提取它们的生物体，这些重复结构表现出微小的结构差异。FCS 及其衍生物的详细表征对于在新型抗凝药物的开发中建立其构效关系非常重要。在本研究中，应用在线亲水相互作用色谱-傅里叶变换质谱（FTMS）分析了四种海参（Isostichopus badionotus、Pearsonothuria graeffei、Holothuria mexicana 和 Acaudina molpadioides）选择性降解产生的 FCS 寡糖。使用糖组学软件包 GlycReSoft 对这些解聚的 FCS 片段进行定量和比较。定量片段主要具有三糖重复组成，不同种类海参的岩藻糖基化（包括其硫酸化）显示出显着差异。对天然FCS多糖的FTMS离子峰和自上而下的核磁共振波谱的详细分析验证了该方法的准确性。因此，定义了来自这些不同海参的 FCS 链的新结构模型。这种自下而上的方法提供了丰富详细的结构分析，并提供了高精度和可重复性的定量信息，应该适用于FCS及其寡糖的质量控制。