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Reduced phagocytosis and killing of Cryptococcus neoformans biofilm-derived cells by J774.16 macrophages is associated with fungal capsular production and surface modification.
Fungal Genetics and Biology ( IF 3 ) Pub Date : 2019-07-26 , DOI: 10.1016/j.fgb.2019.103258
Hiu Ham Lee 1 , Jaclyn Del Pozzo 2 , Sergio A Salamanca 3 , Hazael Hernandez 4 , Luis R Martinez 5
Affiliation  

Cryptococcus neoformans is an opportunistic encapsulated pathogen that causes life-threatening meningoencephalitis in individuals with immunosuppression. We compared the interactions of C. neoformans planktonic and biofilm-derived cells with J774.16 macrophage-like cells. Planktonic cells are more phagocytized and killed by J774.16 cells than biofilm-derived fungal cells. Biofilm-derived cryptococci possess larger capsule size and release significantly more capsular polysaccharide than planktonic cells in culture. Biofilm-derived fungi exhibited upregulation of genes involved in capsular production. Capsular-specific monoclonal antibody 18B7 demonstrated differential binding to the surface of planktonic and biofilm-derived cryptococci providing a plausible strategy for fungal evasion of macrophages and persistence. Future studies are necessary to elucidate how C. neoformans biofilm-derived cells regulate their virulence factors when interacting with cells of the immune system.

中文翻译:

J774.16巨噬细胞减少的吞噬作用和对新隐球菌生物膜来源的细胞的杀伤作用与真菌荚膜的产生和表面修饰有关。

新型隐球菌是一种机会性封装病原体,可在免疫抑制患者中引起危及生命的脑膜脑炎。我们比较了新隐梭菌浮游生物和生物膜来源的细胞与J774.16巨噬细胞样细胞的相互作用。与生物膜来源的真菌细胞相比,浮游生物细胞更易被J774.16细胞吞噬并杀死。生物膜来源的隐球菌比培养的浮游细胞具有更大的胶囊大小,并释放出明显更多的荚膜多糖。生物膜来源的真菌表现出参与荚膜生产的基因上调。胶囊特异性单克隆抗体18B7表现出与浮游生物和生物膜来源的隐球菌表面的不同结合,为真菌逃避巨噬细胞和持久性提供了合理的策略。
更新日期:2019-07-26
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