In this study, we present a fast, simple, low‐cost and disposable method for determination of phenolic content in water samples, using a paper based polyphenol oxidase biosensor. The propylamine functionalized silica nanoparticles was dropped onto a paper sheet. After drying at room temperature, the potato tissue extract including polyphenol oxidase was immobilized on the paper via physical and chemical adsorption. The modified paper was placed on the top of the graphite screen printed electrode. To construct of an electrochemical nanobiosensor, the electrochemical behavior of the modified electrode in different steps was investigated by cyclic voltammetry and electrochemical impedance spectroscopy methods. After being optimized the effective parameters, the changes in the biosensor electrochemical response vs. to the different concentrations of the substrate (phenol solution) were monitored by differential pulse voltammetry and amperometry methods. The linear relationships for phenol detection were obtained in the concentration ranges of 0.01–160 μM and 0.1–300 μM with a detection limit of 0.007 μM and 0.042 μM with DPV and amperometry methods, respectively. This method was successfully used in the voltammetric determination of the phenol content in the real samples, like the river water and the wastewater of wood factory.

中文翻译：

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快速测定水中样品中的酚类化合物：基于纸的纳米生物传感器的开发，该传感器经过功能化二氧化硅纳米颗粒和马铃薯组织的修饰

在这项研究中，我们提出了一种基于纸质的多酚氧化酶生物传感器，用于测定水样中的酚含量的快速，简单，低成本且一次性的方法。将丙胺官能化的二氧化硅纳米颗粒滴到纸上。在室温下干燥后，通过物理和化学吸附将包括多酚氧化酶的马铃薯组织提取物固定在纸上。将改性纸放在石墨丝网印刷电极的顶部。为了构建电化学纳米生物传感器，通过循环伏安法和电化学阻抗谱法研究了修饰电极在不同步骤中的电化学行为。优化有效参数后，生物传感器电化学响应的变化与 通过差分脉冲伏安法和安培法监测不同浓度的底物（苯酚溶液）的浓度。用DPV和安培法分别在0.01-160μM和0.1-300μM的浓度范围内获得了苯酚检测的线性关系，检出限分别为0.007μM和0.042μM。该方法已成功地用于伏安法测定实际样品中的苯酚含量，如河水和木厂废水。