Over the past decade, RNA sequencing (RNA-seq) has become an indispensable tool for transcriptome-wide analysis of differential gene expression and differential splicing of mRNAs. However, as next-generation sequencing technologies have developed, so too has RNA-seq. Now, RNA-seq methods are available for studying many different aspects of RNA biology, including single-cell gene expression, translation (the translatome) and RNA structure (the structurome). Exciting new applications are being explored, such as spatial transcriptomics (spatialomics). Together with new long-read and direct RNA-seq technologies and better computational tools for data analysis, innovations in RNA-seq are contributing to a fuller understanding of RNA biology, from questions such as when and where transcription occurs to the folding and intermolecular interactions that govern RNA function.

中文翻译：

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RNA测序：十几岁。

在过去的十年中，RNA测序（RNA-seq）已成为在转录组范围内分析差异基因表达和mRNA差异拼接的必不可少的工具。但是，随着下一代测序技术的发展，RNA-seq也随之发展。现在，RNA-seq方法可用于研究RNA生物学的许多不同方面，包括单细胞基因表达，翻译（翻译组）和RNA结构（结构组）。人们正在探索令人兴奋的新应用，例如空间转录组学（spatialomics）。结合新的长期阅读和直接的RNA-seq技术以及更好的数据分析计算工具，RNA-seq的创新正在帮助人们更加全面地了解RNA生物学，