Chemical investigation of the Kappaphycus alvarezii-derived endophytic fungus Aspergillus micronesiensis lead to the isolation of three novel dibenzospiroketals, aspermicrones A-C (1-3). Their chemical structures were determined by extensive analysis of HR-ESI-MS and NMR spectral data. The absolute configurations of them were determined by experimental and TD-DFT theoretical calculated circular dichroism spectra. Compound 2 exhibited selective cytotoxic effect toward HepG2 cell line (IC50 = 9.9 µM). Additionally, both of compounds 2 and 3 displayed anti-microbial activity against Staphylococcus aureus (MIC = 123.2 µM for each compound). Compound 1 was inactivity in both cytotoxic and anti-microbial assays.

中文翻译：

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Aspermicrones AC，来自海藻的内生真菌Micronesiensis的新型二苯并螺酮。

化学研究的阿尔伯卡帕氏菌衍生的内生真菌Micronesiensis导致分离出三种新型的二苯并螺酮类化合物，Aspermicrones AC（1-3）。通过对HR-ESI-MS和NMR光谱数据进行广泛分析，确定了它们的化学结构。通过实验和TD-DFT理论计算的圆二色性光谱确定它们的绝对构型。化合物2对HepG2细胞系表现出选择性的细胞毒作用（IC50 = 9.9 µM）。此外，化合物2和3均显示出对金黄色葡萄球菌的抗菌活性（每种化合物的MIC = 123.2 µM）。化合物1在细胞毒性和抗微生物试验中均无活性。