This opinion piece describes a new design for the remediation of recalcitrant biofilms. It builds on previous work to develop engineered E. coli that recognize quorum sensing signals from pathogens in a biofilm and secrete toxins in response. To solve the challenge of dilute signalling molecules, we propose to use nanobodies and enzymes displayed on the surface of the cells to localize them to the biofilm and degrade the extracellular polymeric substances, thus creating a solution with better ‘seek and destroy’ capabilities.

中文翻译：

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BeQuIK（Biosensor Engineered Quorum Induced Killing）：用于破坏顽固生物膜的设计细菌。

这篇评论文章描述了一种用于修复顽固生物膜的新设计。它以之前的工作为基础，开发工程大肠杆菌，该大肠杆菌识别来自生物膜中病原体的群体感应信号，并分泌毒素作为回应。为了解决稀释信号分子的挑战，我们建议使用显示在细胞表面的纳米抗体和酶将它们定位到生物膜并降解细胞外聚合物，从而创造出具有更好“寻找和破坏”能力的解决方案。