The Major Facilitator Superfamily (MFS) is ubiquitous in living organisms and represents the largest group of secondary active membrane transporters. In plants, significant research efforts have focused on the role of specific families within the MFS, particularly those transporting macronutrients (C, N, and P) that constitute the vast majority of the members of this superfamily. Other MFS families remain less explored, although a plethora of additional substrates and physiological functions have been uncovered. Nevertheless, the lack of a systematic approach to analyzing the MFS as a whole has obscured the high diversity and versatility of these transporters. Here, we present a phylogenetic analysis of all annotated MFS domain-containing proteins encoded in the Arabidopsis thaliana genome and propose that this superfamily of transporters consists of 218 members, clustered in 22 families. In reviewing the available information regarding the diversity in biological functions and substrates of Arabidopsis MFS members, we provide arguments for intensified research on these membrane transporters to unveil the breadth of their physiological relevance, disclose the molecular mechanisms underlying their mode of action, and explore their biotechnological potential.

主要促进者超家族（MFS）在活生物体中无处不在，代表了最大的次要活性膜转运蛋白群。在植物中，大量的研究工作集中在MFS中特定家族的作用上，特别是那些构成该超家族绝大多数成员的大量营养素（C，N和P）的运输。尽管尚未发现过多的其他底物和生理功能，但其他MFS系列的研究仍较少。然而，由于缺乏一种系统的方法来分析整个MFS，因而掩盖了这些转运蛋白的高度多样性和多功能性。在这里，我们提出了拟南芥中编码的所有带注释的包含MFS域的蛋白质的系统发育分析。基因组，并提出该转运蛋白超家族由218个成员组成，聚集在22个家族中。在审查有关拟南芥MFS成员的生物学功能和底物多样性方面的可用信息时，我们为这些膜转运蛋白的深入研究提供了论据，以揭示其生理相关性的广度，揭示其作用方式的分子机制，并探索它们的作用机理。生物技术的潜力。