Lonicera japonica (LJ; honeysuckle) is used in traditional folk medicine in Korea and is a rich source of ascorbic acid and phenolic components that are reported to have antioxidant and antibiotic properties. We performed an in vitro experiment to assess the effects of LJ extracts (LJE) on ruminal fermentation. Timothy hay (0.3 g dry matter [DM]) was incubated with buffer, ruminal fluid, and 0%, 3%, 5%, 7%, and 9% LJE. Batch culture fermentation was conducted separately for 12, 24, and 48 hr to determine gas production (GP), ruminal fermentation characteristics, and microbial population characteristics. The effects on GP were generally similar to those on DM degradability, with a linear decrease observed at 9% extract at 24 hr. NH3 -N showed a linear increase with increasing extract concentrations at 12 hr, whereas a decrease was observed at 24 hr. Extract supplementation decreased methane (CH4 ) production at 12, 24, and 48 hr. In addition, the abundance of fibrolytic bacteria and ciliate-associated methanogen was reduced at all concentrations of extracts. These results indicate that LJE have the potential to serve as a ruminal fermentation modifier to suppress CH4 production with minimal effects on nutrient digestion in the rumen.

中文翻译：

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忍冬提取物的添加对体外瘤胃发酵，甲烷排放和微生物种群的影响。

忍冬（LJ；金银花）在韩国用于传统民间医学，是抗坏血酸和酚类成分的丰富来源，据报道它们具有抗氧化剂和抗生素的特性。我们进行了一项体外实验，以评估LJ提取物（LJE）对瘤胃发酵的影响。将蒂莫西·干草（0.3 g干物质[DM]）与缓冲液，瘤胃液和0％，3％，5％，7％和9％LJE孵育。分批培养发酵分别进行12、24和48小时，以确定产气量（GP），瘤胃发酵特性和微生物种群特性。对GP的影响通常与对DM降解性的影响相似，在24小时9％的提取物中观察到线性下降。NH3-N在12小时时随着提取物浓度的增加呈线性增加，而在24小时时减少。在12、24和48小时，补充提取物可减少甲烷（CH4）的产生。此外，在所有浓度的提取物中，溶纤细菌和纤毛虫相关的产甲烷菌的含量都降低了。这些结果表明，LJE具有作为瘤胃发酵改良剂的潜力，可以抑制CH4的产生，而对瘤胃中的营养物质消化的影响最小。