The purpose of this test was to investigate the effect of salvianic acid A (SAA, CAS No. 76822-21-4) on the quality of boar semen during liquid storage at 17°C. The effects of different concentrations of SAA on semen quality and antioxidant capacity were analyzed. Boar semen was diluted with Beltsville Thawing Solution (BTS) containing different concentrations (0, 15, 30, 45, 60, 75 μM of SAA). During the storage period, sperm activity was measured every 24 hr, and plasma membrane integrity, acrosome integrity, total antioxidant capacity (T-AOC), malondialdehyde (MDA) content, and catalase (CAT) activity were measured at 0, 1, 3, and 5 days. The results from our study suggest that different concentrations of SAA have different effects on semen preservation. Semen samples supplemented with SAA showed reduced effects of oxidative stress on sperm compared to the control samples. Supplementation of 30 μM of SAA significantly improved sperm motility, plasma membrane integrity, acrosome integrity, and antioxidant capacity. However, the addition of SAA to the extender was scarcely beneficial to the improvement of results of artificial insemination with boar semen after liquid preservation. Further studies are necessary in order to demonstrate that SAA has good effects on the liquid preservation of semen.

中文翻译：

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在公猪精液补充剂中补充丹酚酸A以改善精液质量和抗氧化能力。

该试验的目的是研究丹参酸A（SAA，CAS号：76822-21-4）对17°C液体储存期间公猪精液质量的影响。分析了不同浓度的SAA对精液质量和抗氧化能力的影响。公猪精液用包含不同浓度（0、15、30、45、60、75μMSAA）的贝尔特维尔解冻溶液（BTS）稀释。在储存期间，每24小时测量一次精子活性，并分别在0、1、3、3、5和3时测量质膜完整性，顶体完整性，总抗氧化能力（T-AOC），丙二醛（MDA）含量和过氧化氢酶（CAT）活性。 ，还有5天。我们研究的结果表明，不同浓度的SAA对精液保存的影响不同。与对照样品相比，补充有SAA的精液样品对精子的氧化应激影响降低。补充30μM的SAA可以显着改善精子活力，质膜完整性，顶体完整性和抗氧化能力。但是，在补充剂中添加SAA几乎不能改善液体保存后用公猪精液进行人工授精的效果。为了证明SAA对精液的液体保存有良好的效果，有必要做进一步的研究。将SAA添加到增量剂中对液体保存后公猪精液的人工授精结果的改善几乎没有好处。为了证明SAA对精液的液体保存有良好的效果，有必要做进一步的研究。将SAA添加到增量剂中对液体保存后公猪精液的人工授精结果的改善几乎没有好处。为了证明SAA对精液的液体保存有良好的效果，有必要做进一步的研究。