The study aimed to evaluate nutrient digestibility and intestine gene expression in the progeny from cows supplemented during gestation and fed diets with or without rumen-protected fat (RPF) in the feedlot. Forty-eight Nellore steers, averaging 340 kg, were housed in individual pens and allotted in a completely randomized design using a 2 × 2 factorial arrangement (dams nutrition × RPF). Cows' supplementation started after 124 ± 21 days of gestation. The feedlot lasted 135 days and diets had the inclusion of zero or 6% of RPF. Digestibility was evaluated by total feces collection. Steers were slaughtered using the concussion technique and samples of pancreas and small intestine were collected immediately after the slaughter to analyze α-amylase activity, and the expression of SLC5A1, CD36, and CCK and villi morphometry. Feeding RPF increased nutrients digestibility (p < 0.01). There was no effect of maternal nutrition on digestibility and α-amylase activity in steers (p > 0.05). Duodenal expression of SLC5A1, CD36, and CCK increased in the progeny from restricted cows. In conclusion, protein restriction during mid to late gestation of dams has long-term effects on small-intestine length and on expression of membrane transporters genes in the duodenum of the progeny. However, maternal nutrition does not affect digestibility in the feedlot.

中文翻译：

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育成期日粮中补充母体蛋白质和添加瘤胃保护脂肪对内罗尔ste牛营养物质消化率和肠道基因表达的影响。

该研究旨在评估在饲喂场中添加或不添加瘤胃保护脂肪（RPF）的母牛，在妊娠期间补充饲料和饲喂日粮的后代中的营养消化率和肠道基因表达。将平均体重为340公斤的48头Nellore ers牛放在单独的围栏中，并采用2×2阶乘布置（大坝营养×RPF）以完全随机的设计分配。妊娠124±21天后开始补充母牛。饲养场持续135天，日粮中的RPF为零或6％。通过收集粪便来评估消化率。使用脑震荡技术对牛进行屠宰，屠宰后立即收集胰腺和小肠样品，以分析α-淀粉酶活性，SLC5A1，CD36和CCK的表达以及绒毛形态。饲喂RPF可以提高养分消化率（p <0.01）。产妇营养对ste牛的消化率和α-淀粉酶活性没有影响（p> 0.05）。受限制的母牛的后代中SLC5A1，CD36和CCK的十二指肠表达增加。总之，在大坝中后期妊娠期间的蛋白质限制对小肠长度和后代十二指肠中的膜转运蛋白基因的表达具有长期影响。但是，母体营养不会影响饲养场的消化率。水坝妊娠中期至后期的蛋白质限制对小肠长度和后代十二指肠中的膜转运蛋白基因表达具有长期影响。但是，母体营养不会影响饲养场的消化率。水坝妊娠中期至后期的蛋白质限制对小肠长度和后代十二指肠中的膜转运蛋白基因表达具有长期影响。但是，母体营养不会影响饲养场的消化率。