Due to their inherent hydrophobic and bioinert nature, synthetic degradable polymer-based membranes show inferior stem cell attachment, proliferation, and even differentiation. To overcome these limitations, bioinspired and osteopromotive polydopamine nanoparticle-incorporated fibrous membranes are developed via a two-step route: pH-induced polymerization of dopamine and co-electrospinning of polycaprolactone (PCL) with polydopamine nanoparticles (PDA NPs). Hybrid membranes with optimized PDA NP content exhibit high quantities of apatite deposition and prominent cytocompatibility (cell attachment, spreading and reproduction) and osteo-differentiation potential (alkaline phosphatase activity, calcium mineralization, and osteogenesis-related genes and protein expression) of human mesenchymal stem cells cultured without any growth factors. Importantly, in vivo assessments using a mouse calvarial critical-sized defect demonstrate that the engineered fibrous membranes remarkably boost bone reconstruction and regeneration. Accordingly, our bioinspired PCL-based hybrid fibrous membranes with robust osteoinductive ability can potentially be utilized as a clinically applicable candidate in guided tissue regeneration applications.

由于其固有的疏水性和生物惰性，合成的可降解聚合物基膜显示出较差的干细胞附着，增殖甚至分化。为了克服这些限制，通过两步途径开发了具有生物启发性和骨促进性的聚多巴胺纳米颗粒纤维膜：pH诱导的多巴胺聚合以及聚己内酯（PCL）与聚多巴胺纳米颗粒（PDA NPs）的共电纺丝。具有优化的PDA NP含量的杂化膜显示出大量的人间质干细胞的磷灰石沉积和突出的细胞相容性（细胞附着，扩散和繁殖）和骨分化潜能（碱性磷酸酶活性，钙矿化以及与成骨相关的基因和蛋白质表达）培养的细胞没有任何生长因子。重要的是，使用小鼠颅盖临界大小的缺损进行的体内评估表明，工程化的纤维膜可显着促进骨骼的重建和再生。因此，我们具有强大骨诱导能力的基于生物启发的PCL基杂化纤维膜可以潜在地用作引导组织再生应用中的临床应用候选物。