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The ethylene response factor SmERF8 regulates the expression of SmKSL1 and is involved in tanshinone biosynthesis in Saliva miltiorrhiza hairy roots
Journal of Plant Physiology ( IF 4.3 ) Pub Date : 2020-01-01 , DOI: 10.1016/j.jplph.2019.153006 Zhenqing Bai 1 , Jiawen Wu 2 , Wenli Huang 3 , Jie Jiao 3 , Chenlu Zhang 3 , Zhuoni Hou 4 , Kaijing Yan 5 , Xuemin Zhang 5 , Ruilian Han 4 , Zongsuo Liang 6 , Xiujuan Zhang 7
Journal of Plant Physiology ( IF 4.3 ) Pub Date : 2020-01-01 , DOI: 10.1016/j.jplph.2019.153006 Zhenqing Bai 1 , Jiawen Wu 2 , Wenli Huang 3 , Jie Jiao 3 , Chenlu Zhang 3 , Zhuoni Hou 4 , Kaijing Yan 5 , Xuemin Zhang 5 , Ruilian Han 4 , Zongsuo Liang 6 , Xiujuan Zhang 7
Affiliation
Saliva miltiorrhiza ethylene response factor (SmERF), predicted to be expressed genome-wide, is the potential regulator of tanshinone biosynthesis. However, few studies have investigated its transcriptional regulation pathways in tanshinone biosynthesis. Here, we report an ethylene response factor (SmERF8), which was screened by the SmKSL1 (a key gene in tanshinone biosynthesis) promoter from the S. miltiorrhiza cDNA library. The SmERF8, highly expressed in S. miltiorrhiza root head, is sensitive to Eth stress, and its protein was enriched in the nucleus. The SmERF8 recognizes the GCC-box in the SmKSL1 promoter. Overexpression and RNAi of SmERF8 in S. miltiorrhiza transgenic hairy roots showed that the tanshinone contents were significantly increased in the overexpression transgenic lines and decreased in RNAi lines. These results suggest that the SmERF8 may be a central activator that regulates the expression of SmKSL1 by binding the GCC-box and then promoting tanshinone biosynthesis. Thus, the SmERF8 may functionally accelerate tanshinone biosynthesis by the transcriptional regulation of its key gene.
中文翻译:
乙烯反应因子SmERF8调控SmKSL1的表达并参与丹参发根丹参酮的生物合成
唾液丹参乙烯反应因子 (SmERF) 预计在全基因组范围内表达,是丹参酮生物合成的潜在调节因子。然而,很少有研究调查其在丹参酮生物合成中的转录调控途径。在这里,我们报告了一种乙烯反应因子 (SmERF8),它是由 S. miltiorrhiza cDNA 文库中的 SmKSL1(丹参酮生物合成中的关键基因)启动子筛选的。SmERF8在丹参根茎中高表达,对Eth胁迫敏感,其蛋白在细胞核中富集。SmERF8 识别 SmKSL1 启动子中的 GCC-box。SmERF8在丹参转基因毛状根中的过表达和RNAi表明,过表达转基因株系中丹参酮含量显着增加,而在RNAi株系中丹参酮含量显着降低。这些结果表明 SmERF8 可能是一种中枢激活剂,它通过结合 GCC 盒然后促进丹参酮生物合成来调节 SmKSL1 的表达。因此,SmERF8 可能通过其关键基因的转录调控在功能上加速丹参酮的生物合成。
更新日期:2020-01-01
中文翻译:
乙烯反应因子SmERF8调控SmKSL1的表达并参与丹参发根丹参酮的生物合成
唾液丹参乙烯反应因子 (SmERF) 预计在全基因组范围内表达,是丹参酮生物合成的潜在调节因子。然而,很少有研究调查其在丹参酮生物合成中的转录调控途径。在这里,我们报告了一种乙烯反应因子 (SmERF8),它是由 S. miltiorrhiza cDNA 文库中的 SmKSL1(丹参酮生物合成中的关键基因)启动子筛选的。SmERF8在丹参根茎中高表达,对Eth胁迫敏感,其蛋白在细胞核中富集。SmERF8 识别 SmKSL1 启动子中的 GCC-box。SmERF8在丹参转基因毛状根中的过表达和RNAi表明,过表达转基因株系中丹参酮含量显着增加,而在RNAi株系中丹参酮含量显着降低。这些结果表明 SmERF8 可能是一种中枢激活剂,它通过结合 GCC 盒然后促进丹参酮生物合成来调节 SmKSL1 的表达。因此,SmERF8 可能通过其关键基因的转录调控在功能上加速丹参酮的生物合成。