Essential oils (EOs) are categorized as having antimicrobial, insecticidal, and antioxidant activities. For their antibacterial activity, EOs are widely studied as alternatives to antibiotics in human and veterinary medicines. The inhibitory and bactericidal activities of three EOs (Cinnamomum zeylanicum, Leptospermum scoparium, and Satureja montana) were determined against Listeria monocytogenes ATCC 7644. The chemical compositions of the EOs were determined through gas chromatography mass spectrometry (GC‐MS). Essential oils were tested alone or in binary and ternary combinations for both their inhibitory and their bactericidal activities. Binary and ternary mixtures were tested through a chequerboard assay, and thus fractional inhibitory and fractional bactericidal concentration indices were obtained in relation to the activity of each EO alone. The chemical composition of each EO had different percentages of the main classes of compounds. EOs showed high levels of monoterpenes (hydrocarbons and oxygenated), sesquiterpenes (hydrocarbons and oxygenated), and phenylpropanoids for winter savory, manuka, and cinnamon, respectively. Several synergistic activities against Listeria monocytogenes were demonstrated in binary and ternary mixtures of the EOs. Mixtures of the EOs tested showed potential for use as antibacterial products; moreover, because of the synergistic activities the mixtures could be used in lower quantities than individual EOs on their own.

中文翻译：

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三种精油（肉桂，麦卢卡和冬季咸味）的抗菌活性及其协同作用，对单核细胞增生性李斯特菌

香精油（EOs）被归类为具有抗菌，杀虫和抗氧化活性。由于其抗菌活性，EOs作为人类和兽药中抗生素的替代品已被广泛研究。确定了三种EOs（肉桂，Leptospermum scoparium和Satureja montana）对单核细胞增生李斯特菌的抑制和杀菌活性。ATCC7644。通过气相色谱质谱法（GC-MS）确定EO的化学组成。单独或以二元和三元组合形式对香精油的抑制和杀菌活性进行了测试。通过棋盘实验对二元和三元混合物进行了测试，因此获得了相对于每种单独的EO活性的部分抑菌分数和部分杀菌浓度指数。每个EO的化学成分在主要类别的化合物中所占的百分比不同。EO分别在冬季咸味，麦卢卡和肉桂中显示出高水平的单萜（碳氢化合物和含氧化合物），倍半萜（碳氢化合物和含氧化合物）和苯丙烷类化合物。针对单核细胞增生李斯特菌的几种协同活性在EO的二元和三元混合物中得到了证明。测试的EOs混合物显示出可用作抗菌产品的潜力；此外，由于具有协同作用，混合物的用量要比单个EO单独使用的量少。