In the past decades, advances in microscopy have made it possible to study the dynamics of individual biomolecules in vitro and resolve intramolecular kinetics that would otherwise be hidden in ensemble averages. More recently, single-molecule methods have been used to image, localize, and track individually labeled macromolecules in the cytoplasm of living cells, allowing investigations of intermolecular kinetics under physiologically relevant conditions. In this review, we illuminate the particular advantages of single-molecule techniques when studying kinetics in living cells and discuss solutions to specific challenges associated with these methods.

中文翻译：

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活细胞中的单分子动力学。

在过去的几十年中，显微镜技术的进步使人们有可能在体外研究单个生物分子的动力学并解决分子内动力学，否则该分子内动力学将隐藏在整体平均值中。最近，单分子方法已用于在活细胞的细胞质中成像，定位和跟踪单独标记的大分子，从而允许在生理相关条件下研究分子间动力学。在这篇综述中，我们阐明了研究活细胞动力学时单分子技术的特殊优势，并讨论了与这些方法相关的特定挑战的解决方案。