Post hatch muscle growth and the repair or regeneration of muscle after myofiber injury is mediated by satellite cells. Satellite cells proliferate, migrate, differentiate, and fuse with growing or regenerating myofibers. The proliferation and differentiation of satellite cells are affected by nutrition, but it is unknown how nutrition impacts satellite cell migration. The objective of the study was to determine the effect of a nutrient restriction on satellite cell migration. Satellite cells from the pectoralis major muscle of 1 and 49-day-old Randombred Control Line 2 turkeys were grown in culture, and migration was measured using a wound healing assay. Nutrient restrictions of 0, 5, 10, and 20% of the standard culture medium were applied starting immediately after scratch or 24 h prior to scratch. Nutrient restrictions of 5 and 20% increased 1 D satellite cell migration at 6 h post scratch compared to 1 D satellite cells with standard culture medium but had no effect after 12 h post scratch. Nutrient restrictions started 24 h prior to scratch increased 1 D satellite cell migration at 6 and 12 h post scratch compared to nutrient restrictions started immediately after scratch. The migration of 49 D satellite cells was not affected by the percentage or timing of the nutrient restriction. These data suggest that nutrition has only a minor effect on the migration of turkey pectoralis major muscle satellite cells. Therefore, the influence of nutrition on satellite cell migration is likely not an important factor for evaluating poultry diet formulations to optimize muscle growth and structure for improved meat protein and fat content as well as meat texture.

中文翻译：

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火鸡卫星细胞的营养限制和迁移。

孵化后肌肉生长和肌纤维损伤后肌肉的修复或再生是由卫星细胞介导的。卫星细胞增殖、迁移、分化并与生长或再生的肌纤维融合。卫星细胞的增殖和分化受营养影响，但营养如何影响卫星细胞迁移尚不清楚。该研究的目的是确定营养限制对卫星细胞迁移的影响。来自 1 日龄和 49 日龄 Randombred Control Line 2 火鸡胸大肌的卫星细胞在培养基中生长，并使用伤口愈合测定法测量迁移。在划伤后立即或划伤前 24 小时开始应用 0、5、10 和 20% 的标准培养基的营养限制。与标准培养基的 1D 卫星细胞相比，5% 和 20% 的营养限制增加了 1D 卫星细胞在 6 小时后划痕后的迁移，但在划痕后 12 小时后没有影响。与划痕后立即开始营养限制相比，划痕前 24 小时开始营养限制增加了划痕后 6 和 12 小时的一维卫星细胞迁移。49 D 卫星细胞的迁移不受营养限制的百分比或时间的影响。这些数据表明，营养对火鸡胸大肌卫星细胞的迁移影响很小。所以，