Reactive oxygen species (ROS) are key regulators of numerous subcellular, cellular, and systemic signals. They function in plants as an integral part of many different hormonal, physiological, and developmental pathways, as well as play a critical role in defense and acclimation responses to different biotic and abiotic conditions. Although many ROS imaging techniques have been developed and utilized in plants, a whole-plant imaging platform for the dynamic detection of ROS in mature plants is lacking. Here we report a robust and straightforward method for the whole-plant live imaging of ROS in mature plants grown in soil. This new method could be used to study local and systemic ROS signals in different genetic variants, conduct phenotyping studies to identify new pathways for ROS signaling, monitor the stress level of different plants and mutants, and unravel novel routes of ROS integration into stress, growth regulation, and development in plants. We demonstrate the utility of this new method for studying systemic ROS signals in different Arabidopsis mutants and wound responses in cereals such as wheat and corn.

活性氧（ROS）是许多亚细胞，细胞和系统性信号的关键调节剂。它们在植物中的作用是许多不同的激素，生理和发育途径的组成部分，并且在防御和适应不同生物和非生物条件的反应中起着关键作用。尽管已经开发了许多ROS成像技术并在植物中使用，但仍缺乏用于动态检测成熟植物中ROS的全植物成像平台。在这里，我们报告了一种在土壤中生长的成熟植物中对ROS进行全植物实时成像的强大而直接的方法。这种新方法可用于研究不同遗传变异中的局部和全身性ROS信号，进行表型研究，以确定ROS信号传导的新途径，监测不同植物和突变体的胁迫水平，并阐明ROS整合入植物胁迫，生长调节和发育的新途径。我们证明了这种新方法在研究不同系统性ROS信号中的实用性拟南芥突变体和谷物（例如小麦和玉米）的伤口反应。