BACKGROUND In 6.5 years of newborn screening for severe combined immunodeficiency in California, 3,252,156 infants had DNA from dried blood spots (DBSs) assayed for T-cell receptor excision circles. Infants with T-cell receptor excision circle values of less than a designated cutoff on a single DBS, 2 DBS samples with insufficient PCR amplification, or known genetic risk of immunodeficiency had peripheral blood complete blood counts and lymphocyte subsets assayed in a single flow cytometry laboratory. Cases in which immune defects were ruled out were available for analysis. OBJECTIVE We sought to determine reference intervals for lymphocyte subsets in racially/ethnically diverse preterm and term newborns who proved to be unaffected by any T-lymphopenic immune disorder. METHODS Effective gestational age (GA) was defined as GA at birth plus postnatal age at the time of sample collection. After determining exclusion criteria, we analyzed demographic and clinical information, complete and differential white blood cell counts, and lymphocyte subsets for 301 infants, with serial measurements for 33 infants. Lymphocyte subset measurements included total T cells, helper and cytotoxic T-cell subsets, naive and memory phenotype of each T-cell subset, B cells, and natural killer cells. RESULTS Reference intervals were generated for absolute numbers and lymphocyte subsets from infants with effective GAs of 22 to 52 weeks. Sex and ethnicity were not significant determinants of lymphocyte subset counts in this population. Lymphocyte counts increased postnatally. CONCLUSION This study provides a baseline for interpreting comprehensive lymphocyte data in preterm and term infants, aiding clinicians to determine which newborns require further evaluations for immunodeficiency.

中文翻译：

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无免疫缺陷的早产和足月新生儿淋巴细胞亚群的参考间隔。

背景技术在加利福尼亚州针对严重的联合免疫缺陷症进行的6.5年新生儿筛查中，对3,252,156例婴儿的干血斑（DBS）DNA进行了T细胞受体切除环的检测。单个DBS上T细胞受体切除环值小于指定截断值的婴儿，PCR扩增不足的2个DBS样品或已知的免疫缺陷遗传风险的婴儿在单个流式细胞仪实验室中测定了外周血全血细胞计数和淋巴细胞亚群。排除了免疫缺陷的病例可用于分析。目的我们试图确定种族/种族不同的早产和足月新生儿的淋巴细胞亚群的参考间隔，这些新生儿被证实不受任何T淋巴细胞免疫障碍的影响。方法有效胎龄（GA）定义为出生时的GA加上样本采集时的出生后的年龄。确定排除标准后，我们​​分析了301名婴儿的人口统计学和临床​​信息，完整和差异性白细胞计数以及淋巴细胞亚群，并对33名婴儿进行了系列测量。淋巴细胞亚群的测量包括总T细胞，辅助和细胞毒性T细胞亚群，每个T细胞亚群的幼稚和记忆表型，B细胞和自然杀伤细胞。结果得出有效间隔为22至52周的婴儿的绝对数量和淋巴细胞亚群的参考区间。性别和种族不是该人群淋巴细胞亚群计数的重要决定因素。出生后淋巴细胞计数增加。